The gradual discovery of functional domains in native enamel matrix proteins has enabled the design of smart bioinspired peptides for tooth enamel mimetics and repair. In this study, we expanded upon the concept of biomineralization to design smaller amelogenin-inspired peptides with conserved functional domains for clinical translation. The synthetic peptides displayed a characteristic nanostr...

Abnormalities of enamel matrix proteins deposition, mineralization, or degradation during tooth development are responsible for a spectrum of either genetic diseases termed Amelogenesis imperfecta or acquired enamel defects. To assess if environmental/nutritional factors can exacerbate enamel defects, we investigated the role of the active form of vitamin A, retinoic acid (RA). Robust expressio...

Enamel anomalies – amelogenesis imperfecta, 1: 14000, several types with different heredity. Most frequent type is AD with hypomineralisation. Enamel matrix is normaly created, but the mineralisation is insufficient. The enamel is soft,peels off. Teeth are yellowbrown. Open bite is common. Reduction of pulp chamber occurs relatively early, so the reconstruction with crowns could be provided bef...

Mice lacking amelogenin (KO) have hypoplastic enamel. Overexpression of the most abundant amelogenin splice variant M180 and LRAP transgenes can substantially improve KO enamel, but only ~40% of the incisor thickness is recovered and the prisms are not as tightly woven as in WT enamel. This implies that the compositional complexity of the enamel matrix is required for different aspects of ename...

Defective enamel formation is the defects occurring at the stages of enamel formation. Amelogenesis imperfect (AI) is a hereditary disorder that causes developmental disturbances in the structure of enamel. Quantitative defects in matrix formation leads to hypoplastic form of Amelogenesis imperfecta. Inadequate mineralization of matrix leads to hypocalcification and hypomaturationvariants. It i...

BACKGROUND Mutations in WDR72 have been identified in autosomal recessive hypomaturation amelogenesis imperfecta (AI). OBJECTIVE to describe a novel WDR72 mutation and report the ultrastructural enamel phenotype associated with a different WDR72 mutation. METHODS A family segregating autosomal recessive hypomaturation AI was recruited, genomic DNA obtained and WDR72 sequenced. Four deciduou...

Dental enamel is the only mineralized tissue of epithelial origin in mammals. The exceptional structural complexity and physical properties of tooth enamel seem to be dependent upon the properties of the protein matrix precursor. Proteins involved in enamel biosynthesis guide hydroxyapatite mineral formation, making the tooth enamel the hardest tissue in the vertebrate body. In dentistry, due t...