نتایج جستجو برای: embryonic stem es cell

تعداد نتایج: 1909995  

Journal: :The EMBO journal 2013
Lan Wang Xianzhuo Zeng Shuai Chen Liya Ding Jian Zhong Jonathan C Zhao Liguo Wang Aaron Sarver Antonius Koller Jizu Zhi Yupo Ma Jindan Yu Junjie Chen Haojie Huang

The Polycomb-repressive complex 2 (PRC2) is important for maintenance of stem cell pluripotency and suppression of cell differentiation by promoting histone H3 lysine 27 trimethylation (H3K27me3) and transcriptional repression of differentiation genes. Here we show that the tumour-suppressor protein BRCA1 interacts with the Polycomb protein EZH2 in mouse embryonic stem (ES) and human breast can...

2017
Natsumi Hagiwara-Chatani Kota Shirai Takumi Kido Tomoatsu Horigome Akihiro Yasue Naoki Adachi Yohei Hirai

Embryonic stem (ES) and induced pluripotent stem (iPS) cells are attractive tools for regenerative medicine therapies. However, aberrant cell populations that display flattened morphology and lose ground-state pluripotency often appear spontaneously, unless glycogen synthase kinase 3β (GSK3β) and mitogen-activated protein kinase kinase (MEK1/2) are inactivated. Here, we show that membrane trans...

2009
Jacob Hanna Styliani Markoulaki Maisam Mitalipova Albert W. Cheng John P. Cassady Judith Staerk Bryce W. Carey Christopher J. Lengner Ruth Foreman Jennifer Love Qing Gao Jongpil Kim Rudolf Jaenisch

Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of blastocysts, whereas epiblast stem cells (EpiSCs) are derived from the post-implantation epiblast and display a restricted developmental potential. Here we characterize pluripotent states in the non-obese diabetic (NOD) mouse strain, which prior to this study was considered “non-permissive” for ES cell derivation. We find ...

Journal: :Molecular and cellular biology 2005
Peili Gu Damien LeMenuet Arthur C-K Chung Michael Mancini David A Wheeler Austin J Cooney

Embryonic stem (ES) cell pluripotency and differentiation are controlled by a network of transcription factors and signaling molecules. Transcription factors such as Oct4 and Nanog are required for self-renewal and maintain the undifferentiated state of ES cells. Decreases in the expression of these factors indicate the initiation of differentiation of ES cells. Inactivation of the gene encodin...

2016
Sachiko Yoshie Yuki Ogasawara Masateru Ikehata Kazuyuki Ishii Yukihisa Suzuki Keiji Wada Kanako Wake Satoshi Nakasono Masao Taki Chiyoji Ohkubo

The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on the embryonic stem cell test (EST). The cells were exposed to 21 kHz IF-MF up to magnetic flux density of 3.9 mT during the cell proliferation process (7 days) or the cell differentiation process (10 days) during which an embryonic body dif...

2010
Nobuyuki Ehira Hiroyuki Oshiumi Misako Matsumoto Takeshi Kondo Masahiro Asaka Tsukasa Seya

Malignant tumor cells often express embryonic antigens which share the expression with embryonic stem (ES) cells. The embryonic antigens are usually encoded by ES cell-specific genes, a number of which are associated with tumorigenesis and/or tumor progression. We examined the expression of ES cell-specific genes in the mouse B16 melanoma cell line to identify the factors promoting tumorigenesi...

Journal: :Cell 2008
Alexander Marson Stuart S. Levine Megan F. Cole Garrett M. Frampton Tobias Brambrink Sarah Johnstone Matthew G. Guenther Wendy K. Johnston Marius Wernig Jamie Newman J. Mauro Calabrese Lucas M. Dennis Thomas L. Volkert Sumeet Gupta Jennifer Love Nancy Hannett Phillip A. Sharp David P. Bartel Rudolf Jaenisch Richard A. Young

MicroRNAs (miRNAs) are crucial for normal embryonic stem (ES) cell self-renewal and cellular differentiation, but how miRNA gene expression is controlled by the key transcriptional regulators of ES cells has not been established. We describe here the transcriptional regulatory circuitry of ES cells that incorporates protein-coding and miRNA genes based on high-resolution ChIP-seq data, systemat...

Journal: :The Biochemical journal 2011
Satoshi Yasuda Tetsuya Hasegawa Tetsuji Hosono Mitsutoshi Satoh Kei Watanabe Kageyoshi Ono Shunichi Shimizu Takao Hayakawa Teruhide Yamaguchi Kazuhiro Suzuki Yoji Sato

An understanding of the mechanism that regulates the cardiac differentiation of pluripotent stem cells is necessary for the effective generation and expansion of cardiomyocytes as cell therapy products. In the present study, we have identified genes that modulate the cardiac differentiation of pluripotent embryonic cells. We isolated P19CL6 cell sublines that possess distinct properties in card...

Journal: :Molecular reproduction and development 2007
Hathaitip Sritanaudomchai Kanok Pavasuthipaisit Yindee Kitiyanant Piengchai Kupradinun Shoukhrat Mitalipov Thanit Kusamran

Embryonic stem (ES) cells derived from mammalian embryos have the ability to form any terminally differentiated cell of the body. We herein describe production of parthenogenetic buffalo (Bubalus Bubalis) blastocysts and subsequent isolation of an ES cell line. Established parthenogenetic ES (PGES) cells exhibited diploid karyotype and high telomerase activity. PGES cells showed remarkable long...

Journal: :Neuron 2009
Asa Abeliovich Claudia A. Doege

The recent description of somatic cell reprogramming to an embryonic stem (ES) cell-like phenotype, termed induced pluripotent stem (iPS) cell technology, presents an exciting potential venue toward cell-based therapeutics and disease models for neurodegenerative disorders. Two recent studies (Dimos et al. and Ebert et al.) describe the initial characterization of neurodegenerative disease pati...

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