Over the past decades, plant cryopreservation technologies have been evolving rapidly, opening the door to the possibility of long-term storage of valuable genetic resources of many crop and forest species. From the original slow-cooling approach, research has moved to easier and more reproducible techniques which allow a complete vitrification of extraand intra-cellular liquids through the dir...

Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease was designed for specific cleavage of an endogenous target sequence adjacent t...

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A nu...

Background: Ferula gummosa Boiss. is a medicinal and economical plant belonging to Apiaceae family. It is a native herb of Iran and is in serious danger of extinction. Objective: Gamma irradiation is an environmental stress and can consider as a new tool to change the content of secondary metabolites in medicinal plants. The effect of gamma irradiation on phenolic content and radical sc...

Abstract The Aceh patchouli plant ( Pogostemon cablin . Benth) is a plantation that produces essential oils with great potential, known as oil. use of in vitro culture techniques on plants needs to be developed meet the seeds. This study aimed analyze effect growth regulators (PGRs) cytokinins 6-Benzyl Amino Purine (BAP) and Thidiazuron (TDZ) auxin 1-Naphthaleneacetic Acid (NAA) organogenesis a...

Cassava (Manihot esculenta) feeds c. 800 million people world-wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA-sequencing and develop an open access, web-based interfa...

BREMNER D.H., MAGILL WJ. DEIGHTON N.& BENSON E.E. 1997. An evaluation of analytical methods for the detection of secondary oxidation products in dedifferentiated plant cultures.-Phyton (Horn, Austria) 37 (3): (39) (44). A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS te...

Epidermal and subepidermal cells in the abaxial, basal region of the maize (Zea mays L.) immature zygotic embryo (IZE) scutellum can be induced by exogenous auxin to proliferate and undergo somatic embryogenesis. Successful genetic transformation of IZEs depends not only on optimizing transformation parameters for these totipotent cells, but also on achieving high embryogenic callus induction f...

Pruacan is a rare medicinal plant endemic to the Dieng Plateau of Central Java, Indonesia which has properties as an aphrodisiac. Increasing genetic variability ofthis in order support breeding programs and increase productivity active ingredients this important. This study aimed evaluate effect chemical mutagen EMS on growth development vitro culture pruacan lethal dose determination. Embryoge...