Chick embryo fibroblasts transformed by Rous sarcoma virus have an increased content of two membrane proteins of molecular weights 78,000 and 95,000. The increased content of the 95,000-dalton protein and the principal increase in the content of the 78,000-dalton protein are not an early consequence of cell transformation but instead are secondary to the rapid depletion of glucose from the gro...

objective(s): juglone is isolated from many species of the juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. here, we evaluated juglone-induced antitumor effect in ovarian cancer skov3 cells. materials and methods: mtt assay was performed to examine juglone anti-proliferative effect. cell cycle and apoptosis were studied using flow cytometry in juglone-tr...

in our search for new anticancer medicinal plants, the antiproliferative activity of the methanol extract of daphne mucronata (thymelaeaceae) was evaluated using human myelogenous leukemia k562 cells. the cells responded to plant treatments in a dose dependent manner and the ic50 of the crude extract (equivalent to 1 g of plant leaves powder per ml) and the purified active component were found ...

humans suffer transient or persistent starvation due to a lack of food intake, either because of fasting, voluntary dieting, or due to the scarcity of available food. at the cellular level it is possible to possess pathological starvation during ischemia and solid tumors. blood provides many nutrients to our cells, and researchers provide these nutrients to cells in culture in the form of enric...

Embryo formation is the first patterning process during vegetative plant growth. Using transposons as insertional mutagens in Arabidopsis, we identified the mutant edd1 that shows embryo-defective development. The insertion mutation is lethal, arresting embryo growth between the globular and heart stages of embryonic development. The mutant phenotype cosegregates with a transposed Dissociation ...

The nudel gene of Drosophila is maternally required both for structural integrity of the egg and for dorsoventral patterning of the embryo. It encodes a structurally modular protein that is secreted by ovarian follicle cells. Genetic and molecular studies have suggested that the Nudel protein is also functionally modular, with a serine protease domain that is specifically required for ventral d...

Background: Auto-immunization was proposed as the mechanism of contralateral testicular damage following unilateral vas deferens injuries. The objective of this experimental in vitro study was to elucidate the effect of tacrolimus (TS) as a powerful immunosuppressant on contralateral epididymal sperm fertilizing capacity following unilateral vas deferens obstruction (UVO) in mice. Materials and...

Background: In the last few years, a marked decrease in male fertility has been reported. Environmental factors were recently suspected for this problem. The misuse of medicines especially antibiotics are among environmental factors that can affect reproductive system. Ciprofloxacin is a synthetic antibacterial agent belonging to the family of fluoroquinolones with a very broad spectrum effect ...

embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...