BACKGROUND The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. METHODS The 398-bp sequence of a gene that code...

There are many concerns about safety of food contaminated with antibacterial residues. This study was designed to investigate the occurrence of chloramphenicol (CAP) residue in broiler chickens tissues, namely liver, kidney and muscle. One hundred and sixty broiler chickens carcasses were collected from three provinces of Iran. Four Plate Test (FTP), ELISA and HPLC were used to qualify and quan...

INTRODUCTION Various methods are used for the diagnosis of visceral leishmaniasis (VL), such as microscopic examination, culture and inoculation of laboratory animals; however, serological assays are commonly used for the detection of antibodies in serum samples with a wide range of specificity and sensitivity. METHODS The purpose of this study was to compare three serological methods, includ...

BACKGROUND Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensitive, however their specificity are not satisfactory. Therefore, WHO recommended specific serological methods using specific antigens, sp...

BACKGROUND Herpes simplex virus (HSV) type 1 and 2 are common infectious agents worldwide. Data on prevalence of HSV-1 and HSV-2 infection are limited in Asia, especially in Iran. The aim of this study was to determine the seroprevalence of HSV type 1 and 2 based on age, gender, marital status, education, living area, job, symptoms and history of disease variables. METHODS The study populatio...

BACKGROUND Toxoplasma gondii is a worldwide obligate intercellular parasite. Felids are its definitive host and warm-blooded animals including humans are its intermediate host. The aim of this seroepidemiological study was to investigate the frequency of human infection using ELISA method and related risk factors in Tabriz City, northwestern Iran. METHODS In this cross sectional study, 171 bl...

Background Chlamydia trachomatis (C. trachomatis) is the main cause of bacterial sexually transmitted infections. In women, this infection can lead to tubal infertility. Objective In this study we investigated C. trachomatis among infertile and fertile women with both polymerase chain reaction (PCR) and ELISA methods in Ahvaz, Iran. Materials and Methods This case-control study was conducte...

BACKGROUND Hepatitis delta virus (HDV) is dependent on the hepatitis B virus for transmission and propagation. Based on isolated HDV sequences from different parts of the world, at least three major different genotypes with different geographic distributions are suggested. Studies have shown that genotype 1 is the predominant genotype of HDV in different parts of Iran; however, the genotype dis...

Cucurbit yellow stunting disorder virus (CYSDV) is a destructive virus of cucurbits in Iran. During 2008-2012 growing seasons a total of 366 cucurbit samples including melon, cucumber, snakemelon and squash with typical symptoms of CYSDV infection were collected from ten Provinces in Iran. They were screened by ELISA and the infectivity of ELISA-positive samples was confirmed by RT-PCR. The res...

BACKGROUND Cystic echinococcosis (CE) is a zoonotic disease with global prevalence, which causes considerable health problems and economic losses throughout the world. The aim of this study was to assess the seroepidemiology of CE in Doroud City, Lorestan Province, Iran, considered a neglected endemic location. METHODS An ELISA was performed using recombinant AgB from Apr to Jul 2015 in Lores...