A method of fractionation of proteins in solution in which an electrophoretic adaptation of the principles of the Clusius column is utilized was suggested by Kirkwood in 1941 (1) and tested experimentally by Nielsen and Kirkwood (2) several years later. Recently an electrophoresis-convection apparatus of improved design has been described and successfully used in the fractionation of the pseudo...

A method of fractionation of proteins in solution in which an electrophoretic adaptation of the principles of the Clusius column is utilized was suggested by Kirkwood in 1941 (1) and tested experimentally by Nielsen and Kirkwood (2) several years later. Recently an electrophoresis-convection apparatus of improved design has been described and successfully used in the fractionation of the pseudo...

Previous investigators have reported a change in distribution of serum protein fractions, as measured by the areas subtending the conventional electrophoresis curves, as a result of a change in ionic strength of the buffer (1) or as a result of a change in total protein concentration (1, 2). A similar effect of total protein concentration on the electrophoretic analysis of ovalbumin has been sh...

Ghosts prepared in CO(2)-saturated water from unwashed human red cells can be fragmented mechanically, but ghosts from thrice washed cells cannot. If the ghosts are prepared by freezing and thawing, this difference is not observed. The electrophoretic velocity varies also with the way in which the ghosts are prepared. The pH-mobility dependence of washed red cells flatten off to a plateau at pH...

Capillary electrophoresis (CE) is an analytical method that is useful for investigating processes that modify the charge of proteins. This paper explores the ability of CE to rationalize charges and electrophoretic mobilities of a simple protein--insulin and its acylated derivatives--as a function of pH. Insulin is a peptide hormone (MW = 5700) that has two alpha-amino groups (G alpha and F alp...

Ristocetin will induce the agglutination of platelets in the presence of von Willebrand factor. In previous studies, an electrostatic mechanism was proposed for this phenomenon wherein first the platelet's surface charge is reduced by the binding of ristocetin and then the von Willebrand factor acts as a bridge between platelets. To test this hypothesis, the effects of ristocetin and von Willeb...

Continuous free-flow electrophoretic separation has been used to obtain relatively pure preparations of synaptosomes and synaptic vesicles from crude fractions of guinea pig brain homogenates. Measurements of the contents of protein, neuraminic acid, and bound acetylcholine; the activities of succinic dehydrogenase, adenosine triphosphatase, choline acetylase, and 5'-nucleotidase; and the uptak...

Recently zone electrophoretic techniques have been employed to characterize the site of thyroxine-binding in plasma (1-7). In most reports, an association has been described between thyroxine and a protein moiety intermediate in electrophoretic mobility at pH 8.6 between the al and the a, globulins. A concentrated source of this thyroxine-binding protein (TBP) has not been available for further...

The observed mobility ranges for the chief electrophoretic components of plasma and its fractions, as studied under conditions standard for control of large-scale fractionation, fall into two classes : (1) components poor in lipoids, whose characteristic mobilities appear independent of conditions of fractionation, degree of purity and usual conditions and duration of storage; ( 2 ) components ...