Electrografting of aryldiazonium species is useful for linkage between electrochemical transducer surface and biosensor recognition elements. However, the process proceeds uncontrollably to form branched/multilayer growth. This paper reports harnessing steric hindrance controlled growth coupling agent during electrografting screen-printed carbon electrode surface, which was subsequently used CR...

This work reports the development of a simple and rapid electrochemical immunosensor for determination breast cancer biomarker Cancer Antigen 15–3 (CA 15–3). Disposable cost-effective chips, consisting gold screen-printed electrodes (AuSPEs), were used to develop portable devices monitoring in point-of-care (PoC), under clinical context. The biosensor preparation consisted two steps. First, sel...

Silica coated silver (Ag@SiO2) nanoparticles were prepared and characterized by transmission electron microscope (TEM) and UV-vis absorption, and the nanoparticles were used as labels in sandwich-type immunosensor of Escherichia coli O157:H7 (E. coli O157:H7). The labels involved in immunoreaction were dissolved by mixed acid of hydrofluoric acid and nitric acid, and the released Ag(+) ions wer...

Biosensors have been developed for the detection of pesticides using integrated enzymes, antibodies, cell and DNA-based biosensors. Enzymatic determination of pesticides is most often based on inhibition of the activity of selected enzymes such as cholinesterase, acid phosphatase, ascorbate oxidase, acetolactate synthase and aldehyde dehydrogenase. Enzymatic Biosensors were developed using vari...

Background The development of biosensors for the diagnosis and monitoring of diseases, drug discovery, proteomics, and the environmental detection of pollutants and/or biological agents is an extremely significant problem. Fundamentally, a biosensor is derived from the coupling of a ligand-receptor binding reaction to a signal transducer. In this study we used as a model a peptide marker of DEN...