Microbial communities present on eggshell surfaces of wild birds are weakly studied, especially their influence on embryo infection and, thus, egg viability. Bacterial density of wild bird eggshells is very low, and most DNA extraction protocols are frequently unsuccessful. We have efficiently adapted a chelex-based DNA isolation method for 16S ribosomal gene amplification from the total commun...

BACKGROUND The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. MATERIALS AND METHODS In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occa...

this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each...

to determine the effect of different levels of l-glutamine and glycerol on freezing coated sperm, semen were collected from 4 taleshi rams through artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. ejaculates were pooled, centrifuged and supernatant was removed, then cell fractions were used. samples were split into 9 equal parts and 3 (g3), 5 (g5) or 7% (g7) glycerol ...

Supplementation of vitamin E (α-tocopherol) as an antioxidant into extender is useful to maintain sperm quality during the process cryopreservation by protecting sperms from reactive oxygen species (ROS). This study aims evaluate protective impacts supplementation on motility, viability, and reducing abnormality in Sapera goats. The sample semen used present was obtained fresh good-quality ejac...

The present study was conducted to detect sperm apoptosis in fresh, capacitated and frozen ram semen and to determine its relationship with other seminal parameters as motility and viability. The cryopreservation process induces the plasma membrane disruptions, attended with increasing of phoshpatidylserine molecules expression on the extracellular surface, wherefore some of the live spermatozo...

Two contrasting estuarine copepods, Acartia tonsa and Eurytemora carolleeae, the former a broadcast spawner latter brood spawner, were fed constant carbon-based diatom diet, but which had variable N:P content, elemental composition (C, N, P) of tissue eggs, as well changes in rates grazing, excretion, egg production viability measured. To achieve varied Thalassiosira pseudonana was grown contin...

The purpose of this study was to determine the effect adding several levels glutathione in egg yolk coconut water dilution (EY-CW) on quality angus bull semen during storage. material used is fresh from one 3 years old that has good quality. Good dilut with EY-CW diluent addition at 0% (T0), 0.1% (T1), 0.2% (T2), and 0.3% (T3). After dilution, stored 3-5oC. Evaluation after carried out every 24...

The ability to store different insect stadia for prolonged periods provides considerable flexibility and ability to conduct experiments properly. Therefore, studies were undertaken to determine the effect of storage temperature and duration on viability of eggs of Helicoverpa armigera (Hübner). The percentage egg hatch and incubation period were significantly (P=0.01) influenced by egg age, sto...

In this study, effect of increasing temperature on life cycle, mortality, and behavior the mosquito species, Aedes aegypti was evaluated. Heat shock applied at 32°C, 37°C 42°C for 20 minutes, 1 hour 2 hours each to egg, 2nd instar, 3rd instar pupal stage separately changes in cycle pattern recorded a routine basis. The control 27°C which reared. When heat-shock eggs, developmental period found ...