Fluorescent in situ hybridization (FISH) with a 21q11-specific probe (CB21c1) consisting of three non-overlapping cosmids has been applied to interphase amniocytes of pregnancies at increased risk for fetal aneuploidy (N = 78) and to interphase lymphocytes, cultured and uncultured, of patients referred for Down syndrome (N = 19 and 28, respectively). In the uncultured amniocytes, six chromosome...

The enhanced mechanical behavior of polymer nanocomposites with spherical filler particles is attributed to the formation matrix-filler interphases. nano-scale leads particularly high interphase volume fractions while rendering experimental investigations extremely difficult. Previously, we introduced a molecular dynamics-based model capturing crucial spatial profiles elastic and inelastic prop...

We used in situ hybridization with a probe for the X chromosome to study interphase cells of bone marrow and peripheral blood specimens from a male patient with acute lymphoblastic leukemia characterized by hyperdiploidy, including trisomy X. In a posttreatment bone marrow specimen, which was interpreted as a regenerating bone marrow morphologically and which demonstrated a normal karyotype cyt...

The clinical efficacy of evaluating genetic anomalies in metaphase cells versus interphase nuclei for multiple myeloma (MM) is poorly understood. Therefore, survival for 154 patients with newly diagnosed untreated MM was compared with results from analysis of metaphase and interphase cells. Metaphases were studied by conventional cytogenetics and fluorescent-labeled DNA probes (fluorescence in ...

A continuum model for the phase separation and coarsening in electrostatically driven granular media is formulated in terms of a Ginzburg-Landau equation subject to conservation of the total number of grains. In the regime of well-developed clusters, the continuum model is used to derive "sharp-interface" equations that govern the dynamics of the interphase boundary. The model captures the esse...

Using a modified Giemsa-banding technique we have observed what appear to be chromosomes during interphase in nuclei from Allium cepa root tips and Chinese hamster cells (CHO line). During telophase through G1 chromosomes progressively uncoil and decondense. During S chromosomes are comparatively decondensed, but some segments have structure similar to chromosomes in G1 and G2. During G2 the ch...

Fluorescence in situ hybridization makes possible direct visualization of single sequences not only on chromosomes, but within decondensed interphase nuclei, providing a potentially powerful approach for high-resolution (1 Mb and below) gene mapping and the analysis of nuclear organization. Interphase mapping was able to extend the ability to resolve and order sequences up to two orders of magn...

A 2-day method using flow cytometry and FISH for interphase cells was developed to detect monosomy 7 cells in myelodysplastic syndrome patients. The method, Interphase Chromosome Flow-FISH (IC Flow-FISH), involves fixation of leukocytes from blood, membrane permeabilization, hybridization of cellular DNA with peptide nucleic acid probes with cells intact, and analysis by flow cytometry. Hundred...

evanescent wave spectroscopy using thin optically transparent electrodes and multiple reflections allows unique observations of the electrochemical interphase since the region of solution sampled is typically 100 nm from the electrode in the visible spectral region. this technique is found to be highly sensitive to the presence of absorbing adsorbed species