Stanniocalcin-1 (STC-1) is made by kidney collecting duct cells for autocrine and paracrine targeting of nephron cell mitochondria. Here, the ligand stimulates respiratory uncoupling and calcium uniport activity. However, the underlying purpose of these actions and how the renal gene is regulated are poorly understood. In a previous study, we described the time-dependent, stimulatory effects of...

We studied the regulation of intracellular pH (pH i ) in single cultured astrocytes passaged once from the hippocampus of the rat, using the dye 2 9 ,7 9 -biscarboxyethyl-5,6-carboxyfluorescein (BCECF) to monitor pH i . Intrinsic buffering power ( b I ) was 10.5 mM (pH unit) 2 1 at pH i 7.0, and decreased linearly with pH i ; the best-fit line to the data had a slope of 2 10.0 mM (pH unit) 2 2 ...

BACKGROUND Patients with a mild to moderate decrease of glomerular filtration rate (GFR) are at risk of cardiovascular (CV) events and CV remodelling has been demonstrated in patients with advanced chronic kidney disease (CKD). However, early stages of CKD and the mechanisms involved in these modifications have not been studied. METHODS A total of 104 patients with early CKD (mean GFR 60+/-21...

A sensitive and selective ultra-high performance liquid chromatography-tandem mass spectrometric method was developed for the quantification of temozolomide (TMZ) in nonhuman primate (NHP) plasma, cerebrospinal fluid (CSF), and brain extracellular fluid (ECF) following microdialysis. Ethyl acetate was used to extract the plasma and CSF samples, using theophylline as the internal standard (IS). ...

In recent years, a variety of chaos-based cryptosystems have been proposed. Some of these systems are used in designing a pseudo random bit generator (PRBG) for stream cipher applications. Most of the chaotic systems used in cryptography have good chaotic properties like ergodicity, sensitivity to initial values and sensitivity to control parameters. However, some of them are not very suitable ...

The uridine nucleotides uridine-5'-triphosphate (UTP) and uridine-5'-diphosphate (UDP) have previously been identified in media from cultured cells. However, no study to date has demonstrated their presence in brain extracellular fluid (ECF) obtained in vivo. Using a novel method, we now show that UTP and UDP, as well as uridine, are detectable in dialysates of striatal ECF obtained from freely...

Extracytoplasmic function (ECF) σ factors, the largest group of alternative σ factors, play important roles in response to environmental stresses. Tt-RpoE1 is annotated as an ECF σ factor in Thermoanaerobacter tengcongensis. In this study, we revealed that the Tt-tolB gene located downstream of the Tt-rpoE1 gene encoded the cognate anti-σ factor, which could inhibit the transcription activity o...

AIM: to present a systematized management results, to asses the use of a vacuum system in local treatment and to determine factors influencing the outcome of enterocutaneous (ECF) fistulas. DESIGN: Retrospective, descriptive, observational. METHODS: All patients with postoperative ECF from March 1998 to March 2011 were registered. A management protocol by stages was applied. Demographic aspects...

Rothia mucilaginosa is known as a member of commensal bacterial flora in the oral cavity and has received attention as a potential opportunistic pathogen. We previously determined the genomic sequence of R. mucilaginosa DY-18, a clinical strain with biofilm-like structures isolated from an infected root canal of a tooth with persistent apical periodontitis. We found that the DY-18 genome had on...