proteinase 3(pr3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (anca) found in wegener's granulomatosis (wg). we developed a stable expression system for conformationally intact recombinant pr3 (rpr3) in chinese hamster ovary cells (cho-cells). the part of pr3 cdna that encoded the active form of pr3 was s...

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Cyanobacterial HCO3(-) transporters BCT1, SbtA and BicA are important components of cyanobacterial CO2-concentration mechanisms. They also show potential in applications aimed at improving photosynthetic rates and yield when expressed in the chloroplasts of C3 crop species. The present study investigated the feasibility of using Escherichia coli to assess function of a range of SbtA and BicA tr...

background: the over-expression of recombinant proteins in large amount is important for production of therapeutic proteins and structural study. there are several systems for expression of recombinant proteins. one of the most relevant expression systems is escherichia coli (e. coli). although this organism has many advantages, most of recombinant proteins expressed in e. coli hosts form inclu...

background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

Angiogenesis plays an essential role in rapid growing and metastasis of the tumors. Inhibition of angiogenesis is a putative strategy for cancer therapy. Endostatin (Es) is an attractive anti-angiogenesis protein with some clinical application challenges including; short half-life, instability in serum and requirement to high dosage. Therefore, production of recombinant endostatin (rEs) is nece...

Title of Document: Rewiring Quorum Sensing Circuitry for Recombinant Protein Production in E. coli Chen-Yu Tsao, Doctor of Philosophy, 2007 Directed By: Professor William E. Bentley Department of Chemical and Biomolecular Engineering The global objective of this research is to rewire the circuitry of bacterial quorum sensing to facilitate recombinant protein production in bacteria. Previous res...

background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

the µ (mu) opioid receptors, which mediate the effects of morphine, are widely distributed in brain. the purpose of this study was to design a simple expression system for rat µ-receptor in escherichia coli (bl21). in this laboratory study, rat µ-receptor cdna was isolated from pcdna3 vector using xba1 and hind3 restriction enzymes. pet-15b was digested by nco1 restriction enzyme. µ-receptor cd...

escherichia coli (e. coli) bacteria can damage dna of the gut lining cells and may encourage the development of colon cancer according to recent reports. genetic switches are specific sequence motifs and many of them are drug targets. it is interesting to know motifs and their location in sequences. at the present study, gibbs sampler algorithm was used in order to predict and find functional m...