Space is not a boring static stage on which events unfold over time, but a dynamic entity with curvature, fluctuations, and a rich life of its own. Spectacular measurements of the cosmic microwave background, gravitational lensing, type Ia supernovae, large-scale structure, spectra of the Lyman alpha forest, stellar dynamics, and x-ray binaries are probing the properties of spacetime over 22 or...

A methodology is presented for probing silicone-based polymer-surfactant complex films a few nanometers in thickness with atomic force microscopy (AFM). The films include 2D regions of liquid character interspersed in regions of solid character. Images in dynamic regimes of net repulsive compared to net attractive interaction show remarkable differences, and are compared with contact mode image...

Introduction Dynamic Nuclear Polarisation (DNP) has recently been shown to increase the sensitivity of C magnetic resonance spectroscopy (MRS) by several orders of magnitude [1]. The number of metabolites that can be hyperpolarized using this technique is increasing; here we show that [1-C]glutamic acid can also be polarized using DNP. The metabolism of hyperpolarized glutamate to αketoglutarat...

Space is not a boring static stage on which events unfold over time, but a dynamic entity with curvature, fluctuations and a rich life of its own which is a booming area of study. Spectacular new measurements of the cosmic microwave background, gravitational lensing, type Ia supernovae, largescale structure, spectra of the Lyman α forest, stellar dynamics and x-ray binaries are probing the prop...

Single-molecule spectroscopy has developed into a widely used method for probing the structure, dynamics, and mechanisms of biomolecular systems, especially in combination with Förster resonance energy transfer (FRET). In this introductory tutorial, essential concepts and methods will be outlined, from the FRET process and the basic considerations for sample preparation and instrumentation to s...

Probing the dynamic activities of protein kinases in real-time in living cells constitutes a major challenge that requires specific and sensitive tools tailored to meet the particular demands associated with cellular imaging. The development of genetically-encoded and synthetic fluorescent biosensors has provided means of monitoring protein kinase activities in a non-invasive fashion in their n...

Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and...

Photochemically induced dynamic nuclear polarisation (photo-CIDNP) in intact bacterial reaction centres has been observed by 13C-solid state NMR under continuous illumination with white light. Strong intensity enhancement of 13C NMR signals of the aromatic rings allows probing the electronic ground state of the two BChl cofactors of the special pair at the molecular scale with atomic selectivit...

Polymer chains attached by one end to an impenetrable surface at high coverage exemplify a tethered layer of mesoscopic dimensions. At equilibrium, thermal fluctuations of the segment density profile of the brushlike layer reflect the tethered chain dynamics; the probing of these fluctuations by evanescent-wave dynamic light scattering is reported. By utilizing a set of terminally attached laye...