A number of specific duplex DNA sequences which correspond to varying lengths of the lactose operator region have been synthesized by a combination of chemical and enzymatic methods. Repressor binding studies on these synthetic duplex operator molecules show that all the nucleotides essential for full lactose operator-repressor interactions are included in a 17-nucleotide-long duplex DNA that c...

Until recently, arteriography has been the method of choice for diagnosing traumatic aneurysms and for follow-up after transcatheter coil embolization [1-3]. Duplex sonography can now playa complementary role in the diagnosis and followup of these lesions. Serial duplex sonographic examinations provide a noninvasive method of monitoring the cessation of flow within the aneurysms and their resol...

We present a semi-quantitative model of condensation of short nucleic acid (NA) duplexes induced by trivalent cobalt(iii) hexammine (CoHex) ions. The model is based on partitioning of bound counterion distribution around single NA duplex into "external" and "internal" ion binding shells distinguished by the proximity to duplex helical axis. In the aggregated phase the shells overlap, which lead...

Background and Purpose We compared two-dimensional time-of-flight magnetic resonance angiography (MRA) and duplex ultrasonography with arteriography for the detection of 70% to 99% stenoses at the carotid artery bifurcation (ie, surgical disease according to findings of the North American Carotid Endarterectomy Trial). Methods Three blinded readers independently measured stenoses on MRA in 73 v...

We consider a general multiple antenna network with multiple sources, multiple destinations and multiple relays in terms of the diversity-multiplexing tradeoff (DMT). We examine several subcases of this most general problem taking into account the processing capability of the relays (half-duplex or full-duplex), and the network geometry (clustered or non-clustered). We first study the multiple ...

Human genomic samples are complex and are considered difficult to assay directly without denaturation or PCR amplification. We report the use of a base-specific heteropolymeric triplex, formed by native duplex genomic target and an oligonucleotide third strand probe, to assay for low copy pathogen genomes present in a sample also containing human genomic duplex DNA, or to assay human genomic du...

The T4 gene 41 protein and the gene 61 protein function together as a primase-helicase within the seven protein bacteriophage T4 multienzyme complex that replicates duplex DNA in vitro. We have previously shown that the 41 protein is a 5' to 3' helicase that requires a single-stranded region on the 5' side of the duplex to be unwound and is stimulated by the 61 protein (Venkatesan, M., Silver L...

Targeting the interface between DNA quadruplex and duplex regions by small molecules holds significant promise in both therapeutics nanotechnology. Herein, a new pharmacophore is reported, which selectively binds with high affinity to quadruplex–duplex junctions, while presenting poorer for G-quadruplex or alone. Ligands complying reported exhibit selectivity including one observed HIV-1 LTR-II...

the success rate of embryo production critically depends on several factors. one of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (ivf) and in vitro embryo culture (ivc) (1, 2). besides the technique selected for ivf / in vitro embryo production (ivep), there is an important consideration called ivf/ivep culture dishes disinfection (3). for many years...

viability of probiotic bacteria in food during maintenance and time of consuming in food has become a challenge in food hygiene and technology and is important for representing their beneficial health effects. the aim of this study was to determine the survival of probiotic enterococcus faecium derived from koopeh cheese added to industrial iranian ultra-filtrated (uf) cheese and screening for ...