Dormant Bacillus subtilis spores germinate in the presence of particular nutrients called germinants. The spores are thought to recognize germinants through receptor proteins encoded by the gerA family of operons, which includes gerA, gerB, and gerK. We sought to substantiate this putative function of the GerA family proteins by characterizing spore germination in a mutant strain that contained...

Polyunsaturated fatty acyl (PUFA) chains in both the ω3 and ω6 series are essential for normal animal brain development, and cannot be interconverted to compensate for a dietary deficiency of one or the other. Paradoxically, a dietary ω3-PUFA deficiency leads to the accumulation of docosapentaenoate (DPA, 22:5ω6), an ω6-PUFA chain that is normally scarce in the brain. We applied a high-precisio...

Different electronic configurations of a series of trinuclear heterometallic chains with 22 metallic electrons, MM'M(dpa)(4)X(2) (M = Co, Rh; M' = Ni, Pd; X = Cl, NCS), have been modelled in search of new systems with novel electrical properties. For this purpose, we explore the possibility of obtaining low-spin (extensively closed-shell) states by introducing chemical changes to the reference ...

We have developed a visual microwell plate assay for rapid, high-throughput screening for membrane-disrupting molecules such as de novo designed pore formers, antibiotic peptides, bacterial toxins, and lipases. The detectability is based on the strong fluorescence emission of the lanthanide metal terbium(III) (Tb(3+)) when it interacts with the aromatic chelator dipicolinic acid (DPA). While Tb...

Fluorine-18 labelled N,N-diethyl-2-(2-[4-(2-fluoroethoxy)phenyl]-5,7-dimethylpyrazolo[1,5-α]pyrimidine-3-yl)acetamide ([F] DPA-714) binds to the 18-kDa translocator protein (TSPO) with high affinity. The aim of this initial methodological study was to develop a plasma input tracer kinetic model for quantification of [F]DPA-714 binding in healthy subjects and Alzheimer’s disease (AD) patients, a...

SUMMARY In the execution on a smart card, side channel attacks such as the simple power analysis (SPA) and the differential power analysis (DPA) have become serious threat. Side channel attacks monitor the side channel information such as power consumption and even exploit the leakage information related to power consumption to reveal bits of a secret key d although d is hidden inside a smart c...

Factor analysis is widely used in many application areas. The first step, choosing the number of factors, remains a serious challenge. One of the most popular methods is parallel analysis (PA), which compares the observed factor strengths to simulated ones under a noise-only model. This paper presents a deterministic version of PA (DPA), which is faster and more reproducible than PA. We show th...

Cotton fibers are the seed trichomes that developed around and used to make clothes yarn for textile industry. Expression profiling of cotton fiber genes is very important estimate differential gene expression level at different developmental stages. analysis developing enhance length cotton. The three families in five stages (0, 5, 10, 15 20 DPA) tissues was carried out through real-time PCR. ...

Considerable research supports cardiovascular benefits of consuming omega-3 PUFA, also known as (n-3) PUFA, from fish or fish oil. Whether individual long-chain (n-3) PUFA have shared or complementary effects is not well established. We reviewed evidence for dietary and endogenous sources and cardiovascular effects on biologic pathways, physiologic risk factors, and clinical endpoints of EPA [2...

Differential Power Analysis (DPA) is commonly used to obtain information about the secret key used in cryptographic devices. Countermeasures against DPA can cause power traces to be misaligned, which reduces the effectiveness of DPA. Principal Component Analysis (PCA) is a powerful tool, which is used in different research areas to identify trends in a data set. Principal Components are introdu...