Infection of JLS V-9 (mouse bone marrow) cells with Moloney murine leukemia virus leads to appearance of virus-specific DNA in the low molecular weight DNA fraction from infected cells. Most of this DNA is double-stranded. Two discrete forms of double-stranded DNA, both of molecular weight 6 times 10-6, have been characterized. One of these forms is closed-circular, supercoiled DNA; the other f...

Previous research has indicated a qualitative resemblance between the current distribution along actively corroding crevice walls and currents measured in corresponding anodic polarization plots. Using detailed measurements of the potential gradient along the crevice, a semi-quantitative current distribution was calculated for the crevice wall using a double numerical differentiation method. Th...

Objectives: The aim of this study was to assess the effects of different orthodontic vertical loop height and design on its force characteristics at different amount of loop activation by means of finite element method (FEM) analysis. Materials andMethods: Four 3D FEM models were designed of double vertical open loop (DVOL) and double vertical helical open loop (DVHOL) each of them in two heigh...

The input impedance of a large circular loop antenna with arbitrary orientation in a cold magnetoplasma is calculated by using a transmission line theory. New impedance resonances for antennas of finite size in a magnetoplasma in the frequency region below and near the electron cyclotron frequency are indicated theoretically. The resonance peak of the impedance at the lower hybrid resonance fre...

Wild-type and gene 3 mutant filamentous phage stocks, containing different relative amounts of multiple-length particles, were treated exhaustively with DNase and then were highly purified. The phage DNA was extracted and examined by electron microscopy. In all cases, about 0.03% of the molecules were circular dimers. (3)H-labeled phage DNA was separated as to size by sedimentation in a preform...

This thesis proposes a minimum distance diagram of undirected double-loop, which makes use of the cyclic graph's excellent properties on the node number and path length to achieve high performance. Main issues include the method to build the minimum distance diagram of undirected double-loop networks, the algorithm to calculate its diameter, and the step to build optimal undirected double-loop ...

objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

A polyguanine/polycytosine (polyG/polyC) tract in the proximal promoter of the vascular endothelial growth factor (VEGF) gene is essential for transcriptional activation. The guanine-rich (G-rich) and cytosine-rich (C-rich) strands on this tract are shown to form specific secondary structures, characterized as G-quadruplexes and i-motifs, respectively. Mutational analysis of the G-rich strand c...