Potato (Solanum tuberosum L.) has a tetraploid genome. To make mutant lacking specific gene function, it is necessary to introduce mutations into all four alleles. achieve this goal, we developed powerful genome editing tool, CRISPR/dMac3-Cas9, which installed the translation enhancer dMac3 that greatly increased of downstream open reading frame. The CRISPR/dMac3-Cas9 system employing three gui...

Objective(s) Diabetes mellitus is associated with disturbances of learning and memory and cognitive functioning. Aegle marmelos Corr. from Rutaceae family is widely used in Iranian folk medicine for the treatment of diabetes mellitus. Considering the beneficial antidiabetic and antioxidant potential of A. marmelos, this study was conducted to evaluate the effect of oral administration of A. ma...

Fig. 18 Shown are the regulatory elements characterized so far, which control the transcription of the tyrosine aminotransferase (TAT) gene. The TAT gene may be induced either by glycocorticoids or by cyclic adenosine monophosphate (cAMP), which activates the protein kinase A. All distances are given in kilobases (kb) and refer to the starting point of the TAT gene transcription (arrow). mediat...

Rationale: ANP (atrial natriuretic peptide) and BNP (B-type peptide), encoded by the clustered genes Nppa Nppb , are important prognostic, diagnostic, therapeutic proteins in cardiac disease. The spatiotemporal expression pattern stress-induction of tightly regulated, possibly involving their coregulation an evolutionary conserved enhancer cluster. Objective: To explore physiological functions ...

background: to suggest south india ct diagnostic reference levels (drls) by collecting radiation doses for the most commonly performed ct examinations. materials and methods: a pilot study investigated the most frequent ct examinations. 110 ct sites were asked to complete a survey booklet to allow the recording of ct parameters for each of 3 ct examinations during a 1 year time period. dose dat...

A recent study visualizes nascent RNAs in live Drosophila embryos to establish a connection between enhancer strength and the frequency of transcriptional bursts. Interestingly, one enhancer can simultaneously activate two core promoters, challenging models of enhancer-core-promoter communication via direct protein-protein contacts.

The avian leukosis virus (ALV) long terminal repeat (LTR) contains a compact transcription enhancer that is active in many cell types. A major feature of the enhancer is multiple CCAAT/enhancer element motifs that could be important for the strong transcriptional activity of this unit. The contributions of the three CCAAT/enhancer elements to LTR function were examined in B cells, as this cell ...

The 5'-flanking sequence of the human prothrombin gene was isolated by screening a human liver phage library with a human prothrombin cDNA as a hybridization probe. A phage was identified that contained 3 kilobase pairs of DNA upstream of the initiator methionine codon. Primer extension studies showed that the major transcription initiation sites were located 23 and 36 base pairs upstream of th...