This article describes the sequence-specific isolation and purification of intact double-stranded DNA (dsDNA) by oligonucleotide/PNA-assisted affinity capture (OPAC). The OPAC assay is based on selective tagging of a DNA duplex by biotinylated oligodeoxyribonucleotide (ODN) through formation of a so-called PD-loop. The PD-loop is assembled with the aid of a pair of PNA “openers”, which allow se...

UNLABELLED PREMISE OF THE STUDY The chemical diversity of land plants ensures that no single DNA isolation method results in high yield and purity with little effort for all species. Here we evaluate a new technique originally developed for forensic science, based on MagnaCel paramagnetic cellulose particles (PMC), to determine its efficacy in extracting DNA from 25 plant species representin...

Epstein-Barr virus (EBV) reactivation and cell-mediated immune (CMI) responses were followed in 16 Antarctic expeditioners during winter-over isolation at 2 Australian National Antarctic Research Expedition stations. Delayed-type hypersensitivity (DTH) skin testing was used as an indicator of the CMI response, that was evaluated 2 times before winter isolation and 3 times during isolation. At a...

This study aimed to determine Bangkok chicken’sphenotypic characteristics and growth hormone genes using the PCR-RFLP method. The samples used were 50 chickens. Research in field is taking phenotypic traits data. laboratory carried out by DNA isolation, amplification, restriction. showed that DOC weight,body weight, bodyweight gain of DOC-1 month, 1-2 months, 2-3 months chickens significantly d...

Laser micro-dissection permits isolation groups of cells from heterogeneous tissue sections under direct microscopic visualization. This technology not only allows isolation of homogenous subpopulation of cells but also allows immunophenotypically similar cells for RNA/ DNA or protein extraction for downstream applications like western blot, mass spectrophotometry, real time quantitative polyme...

A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator el...

Research into and identification of Mycobacterium tuberculosis can take on a number of facets, many of which involve the use of DNA at one stage or another. The quality and quantity of DNA required will depend on the end-use requirement. For example, good yields of pure, high-molecular-weight DNA uncontaminated by DNA from other sources (i.e., homogeneous) are optimal for the generation of cosm...

Mature muscle has a unique structure that is amenable to live cell imaging. Herein, we describe the experimental protocol for expressing fluorescently labeled proteins in the flexor digitorum brevis (FDB) muscle. Conditions have been optimized to provide a large number of high quality myofibers expressing the electroporated plasmid while minimizing muscle damage. The method employs fluorescent ...