Quantitative levels of indole-3-acetic acid (IAA) were determined in leaf blades of two sugarbeet cultivars by a double standard isotope dilution assay using column chromatography followed by reverse phase C(18) high performance liquid chromatography and gas-liquid chromatography with nitrogen thermionic detection. The double standard method was validated as a quantitative tool by gas chromatog...

In this study, it was aimed to determine the anti-bacterial activities of plant essential oils such as thyme, rosemary, sage, linden, black seed, bay leaf, peppermint, parsley, cinnamon and oleagen on vancomycin resistant enterococci (VRE) pathogenic microorganisms clinical importance. inhibition zones extract 10 plants prepared at 2.5 5 mg / mlconcentrations VRE various were detected by Disk D...

This work describes the application of a pressure-enhanced liquid chromatography (PE-LC) setup to tune separation various large molecules comprised nucleic acids (oligonucleotides, messenger ribonucleic acid [mRNA], and deoxyribonucleic [DNA]). When adding pressure as method development parameter, it indeed becomes possible modify retention, selectivity, peak width. As an example, oligonucleoti...

results under the above chromatography condition, biuret compound (peqb) and the internal standard were detected at 4.5 and 13.5 minutes, respectively. limit of quantitation of the peqb was 0.1 µg/ml. accuracy of the method over the concentration range of 0.1-100 µg/ml was between 88-109%. inter- and intraday precisions were 4-19% and 6-8%, respectively. a good relationship in the form of a pow...

A significantly improved DNA polymerase fidelity assay, based on a gapped plasmid containing the lacZα reporter gene in a single-stranded region, is described. Nicking at two sites flanking lacZα, and removing the excised strand by thermocycling in the presence of complementary competitor DNA, is used to generate the gap. Simple methods are presented for preparing the single-stranded competitor...

Opines are low-molecular-weight metabolites specifically biosynthesized by agrobacteria-transformed plant cells when plants struck crown gall and hairy root diseases, which cause uncontrolled tissue overgrowth. Transferred DNA is sustainably incorporated into the genomes of transformed cells, so that opines constitute a persistent biomarker infection pathogenic agrobacteria can be targeted for ...

The technique of 32P postlabeling of DNA-carcinogen adducts is a useful and extremely sensitive method of detecting and quantitating DNA damage by carcinogens. We have adapted the 32P method to analysis by high-pressure liquid chromatography, making the procedure more rapid and convenient than when thin-layer chromatography is used. Following DNA isolation and hydrolysis, nucleotide-carcinogen ...

A human cDNA encoding a previously unrecognized DNA ligase IV has been identified (Wei, Y.-F., Robins, P., Carter, K., Caldecott, K., Pappin, D. J. C., Yu, G.-L., Wang, R.-P., Shell, B. K., Nash, R. A., Schär, P., Barnes, D. E., Haseltine, W. A., and Lindahl, T. (1995) Mol. Cell. Biol. 15, 3206-3216). Antibodies have been raised against predicted peptide sequences of DNA ligase IV and used to i...

The early region of the polyoma genome encodes three T antigens. We have analyzed the organization of the coding regions for the T antigens, using the nucleotide sequence of polyoma DNA and peptides derived from purified, radio-labeled T antigens, separated by two-dimensional electrophoresis and chromatography. We compared the peptides, predicted from the nucleotide sequence of the DNA, with th...

Two nonmitochondrial forms of DNA polymerase are demonstrated in regenerating rat liver and Morris hepatomas 7777, 7800, and 7794A. The enzymes have been partially purified from subcellular fractions of these tissues. The purification procedure involves fractionation with ammonium sulfate and chromatography on diethylaminoethyl cellulose and on phosphocellulose. The enzymes have different physi...