Escherichia coli NikR regulates cellular nickel uptake by binding to the nik operon in the presence of nickel and blocking transcription of genes encoding the nickel uptake transporter. NikR has two binding affinities for the nik operon: a nanomolar dissociation constant with stoichiometric nickel and a picomolar dissociation constant with excess nickel [Bloom, S. L., and Zamble, D. B. (2004) B...

BACKGROUND In light of the large number of chemicals that are potential developmental neurotoxicants, there is a need to develop rapid screening techniques. OBJECTIVES We exposed undifferentiated and differentiating neuronotypic PC12 cells to different organophosphates (chlorpyrifos, diazinon, parathion), a carbamate (physostigmine), an organochlorine (dieldrin), and a metal (divalent nickel;...

Andrew, Angeline S., Linda R. Klei, and Aaron Barchowsky. Nickel requires hypoxia-inducible factor-1a, not redox signaling, to induce plasminogen activator inhibitor-1. Am J Physiol Lung Cell Mol Physiol 281: L607–L615, 2001.—Human epidemiological and animal studies have associated inhalation of nickel dusts with an increased incidence of pulmonary fibrosis. At the cellular level, particulate n...

N ickel is a potentially allergenic material. It is one of the most common causes of allergic contact dermatitis, particularly in women. l-5 Nickel can also be considered an occupational allergen. Nickel contact dermatitis is common among industrial workers, especially in those industries that use nickel as a raw material. It has been demonstrated that U.S. S-cent coins contain approximately 25...

Combinations of chemical and genetic approaches were used to study the function of divalent metal ions in cleavage of RNA by the ribozyme RNase P RNA. We show that different divalent metal ions have differential effects on cleavage site recognition and rescue of cleavage activity by mixing divalent metal ions that do not promote cleavage by themselves. We conclude that efficient and correct cle...

Acid-volatile sulfides (AVS) are strongly associated with the bioavailability of some divalent metals such as cadmium, copper, lead, nickel and zinc. However, global spatial variability AVS for aquatic systems is unknown. The specific goals this study were to: (1) summarize all available monitoring data from types freshwater saltwater waterbodies (streams/creeks, rivers, lakes/ponds/reservoirs ...

The distribution of counterions in solutions of high molecular mass hyaluronic acid, in near-physiological conditions where mono- and divalent ions are simultaneously present, is studied by small angle neutron scattering and anomalous small angle x-ray scattering. The solutions contain either sodium or rubidium chloride together with varying concentrations of calcium or strontium chloride. The ...

Nickel was measured by atomic absorption spectrometry in three-day collections of feces from healthy hospital workers (age 22-65) who had lived for more than a year ‘in Hartford, Connecticut. None of the 10 subjects (4 , 6j9.) had occupational exposure to nickel. Fecal nickel averaged 3.3 gzg/g (wet weight); SD, ±0.8; and range, 2.1 to 4.4 tg/g. Corresponding figures on a dry-weight basis were ...