Interpretive zone size breakpoints for diffusion tests with 30-micrograms cefotiam disks are diameters of greater than or equal to 18 mm for susceptible and less than or equal to 14 mm for resistant strains. The standard control strains Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 should both give zones 27 to 33 mm in diameter. Tests with 579 clinical isolates yielded an una...

INTRODUCTION Antifungal susceptibility testing assists in finding the appropriate treatment for fungal infections, which are increasingly common. However, such testing is not very widespread. There are several existing methods, and the correlation between such methods was evaluated in this study. METHODS The susceptibility to fluconazole of 35 strains of Candida sp. isolated from blood cultur...

the antimicrobial activity of methanolic extracts from different parts of 11 indigenous wild plantspecies used in traditional medicines of iran were tested against nine species of microorganisms: escherichiacoli, staphylococcus aureus, staphylococcus epidermis, pseudomonas aeruginosa, klebsiella pneumoniae,salmonella typhi, bacillus subtilis, aspergillus niger and candida albicans. the antimicr...

The Vitek GPS-TA card (Vitek Systems, Hazelwood, Mo.) was compared with single-concentration broth microdilution and disk diffusion methods using high-content disks for the detection of high-level resistance to gentamicin and streptomycin in 99 isolates of enterococci (81 Enterococcus faecalis isolates and 18 Enterococcus faecium isolates). The GPS-TA card accurately detected high-level resista...

A total of 319 clinical isolates were compared for amikacin susceptibility by AutoBac I and Bauer-Kirby disk diffusion methods. There was an overall agreement of 98.5% between AutoBac I and Bauer-Kirby. An additional 23 strains resistant to amikacin, obtained from Bristol Laboratories, registered a 65% overall agreement. The sensitivity readings differed between the two methods for 13 organisms...

Cefoperazone is a new cephalosporin with a very wide spectrum of activity, including activity against Pseudomonas aeruginosa. It has less activity on enterococci and Acinetobacter. Of the 459 selected bacterial strains tested in this study, only 1.5% (7 strains and 6 genera) had minimum inhibitory concentrations of >128 ,ug/ml. For a minimum inhibitory concentration breakpoint of <32 p.g/ml (su...

A 5-h broth disk test, read visually for growth or no growth to determine resistance of coagulase-negative staphylococci to 1.6 micrograms of novobiocin per ml, was evaluated as a rapid test for the presumptive identification of Staphylococcus saprophyticus. The correlation with an overnight disk diffusion test was 100%.

Mannitol salt agar was evaluated for detection of oxacillin resistance in 136 Staphylococcus aureus isolates. All mecA-positive isolates (n = 54) were correctly categorized as oxacillin resistant by the disk diffusion test (1-microgram disk; zone diameter, < 16 mm); the specificity was 97.6%. Agar screening (2 micrograms of oxacillin per ml) revealed a sensitivity of 98.1% and a specificity of ...

One hundred four Enterobacter isolates were tested by standard CLSI disk diffusion methods for detecting extended-spectrum beta-lactamases (ESBLs) and with cefepime-clavulanate disk combinations. SHV-12 was produced by 8.7% of isolates. The cefepime-clavulanate combination provided 88% sensitivity and 91% specificity for the detection of SHV-12 ESBL.