The nuclear envelope consists of two membranes traversed by nuclear pore complexes. The outer membrane is continuous with the endoplasmic reticulum. At mitosis nuclear pore complexes are dismantled and membranes disperse. The mechanism of dispersal is controversial: one view is that membranes feed into the endoplasmic reticulum, another is that they vesiculate. Using Xenopus egg extracts, nucle...

Timely spindle disassembly is essential for coordination of mitotic exit with cytokinesis. In the budding yeast Saccharomyces cerevisiae, the microtubule-associated protein She1 functions in one of at least three parallel pathways that promote spindle disassembly. She1 phosphorylation by the Aurora kinase Ipl1 facilitates a role for She1 in late anaphase, when She1 contributes to microtubule de...

Indirect immunofluorescent staining with an antitubulin antibody was used for studying the role of ATP in the regulation of cytoplasmic microtubule disassembly. Depletion of the cellular ATP pool in cultured mouse fibroblasts with various inhibitors of energy metabolism leads to inhibition of the microtubule disassembly induced by colcemid or vinblastine. Glucose added to the inhibitor-containi...

Flexible multisensorial systems are a very important issue in the current industry when disassembling and recycling tasks have to be performed. These tasks can be performed by a human operator or by a robot system. In this paper a robotic system to perform the required tasks is presented. This system takes into consideration the distribution of the necessary tasks to perform the disassembly of ...

Product functions, assembly and disassembly processes largely depend on the connections that are holding the product’s components together. In general, the disconnecting process of established connections is sub-optimal, which often remains unnoticed until maintenance or recycling requires disassembly work. Sometimes connections were optimised to improve the disconnecting processes, but usually...

For environmentally conscious and sustainable manufacturing, many more manufacturers are acting to recycle and remanufacture their post-consumed products. The most critical process of remanufacturing is disassembly, since it allows for the selective extraction of the valuable components and materials from returned products to reduce the waste disposal volume. It is, therefore, important to desi...

The azaquinone-methide elimination is a powerful and efficient reaction useful for disassembly of spacers in prodrug systems. We and others have used the spacer-technique to develop dendritic and polymeric self-immolative molecular systems that can disassemble through a domino-like mechanism upon a stimulus event. In this report, we study the disassembly of a system that can disintegrate throug...

A proper balance between synapse assembly and disassembly is crucial for the formation of functional neuronal circuits and synaptic plasticity in the adult brain. During development, synaptogenesis generates a vast excess of synapses, which are subsequently eliminated. Importantly, aberrant synaptic disassembly during development underpins many neurological disorders. Wnt secreted proteins are ...