The DEAH-box NTPase Prp43 disassembles spliceosomes in co-operation with the cofactors Ntr1/Spp382 and Ntr2, forming the NTR complex. How Prp43 is regulated by its cofactors to discard selectively only intron-lariat spliceosomes (ILS) and defective spliceosomes and to prevent disassembly of earlier and properly assembled/wild-type spliceosomes remains unclear. First, we show that Ntr1΄s G-patch...

Although mammals are thought to lose their capacity to regenerate heart muscle shortly after birth, embryonic and neonatal cardiomyocytes in mammals are hyperplastic. During proliferation these cells need to selectively disassemble their myofibrils for successful cytokinesis. The mechanism of sarcomere disassembly is, however, not understood. To study this, we performed a series of immunofluore...

Actin-interacting protein 1 (AIP1) is a conserved WD repeat protein that promotes disassembly of actin filaments when actin-depolymerizing factor (ADF)/cofilin is present. Although AIP1 is known to be essential for a number of cellular events involving dynamic rearrangement of the actin cytoskeleton, the regulatory mechanism of the function of AIP1 is unknown. In this study, we report that two ...

In this work, we revisited the disassembly approach (also known as Zelder's approach) recently proposed for sensing pyrophosphate (PPi) in water and based on decomposition of metal-salen complexes. A systematic study devoted to structural optimization novel class PPi-responsive fluorogenic probes was conducted. Screening eight different vicinal diamines ( i.e. , bridge salen ligand) combined wi...

Abstract In 2021, sales of electric bicycles in Germany increased compared to the previous year, leading a 43% share total German bicycle market. The Bicycle Industry Association (ZIV) expects this growth continue and that by 2025 every second sold will have an motor. Therefore, due increasing number sales, both built-in resources possible treatments after use phase need be considered regarding...

Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer. FACT mediates transcription-linked nucleosome disassembly and also nucleosome reassembly in the wake of the RNA polymerase II transcription complex, and in this way maintains the repression of 'cryptic' promoters found within som...

We investigated the role of cortical actin filaments (F-actin) in the regulation of PRL secretion in cultured normal anterior pituitary cells. F-actin dynamics were evaluated by fluorescence microscopy, and PRL secretion from attached cells was measured by the reverse hemolytic plaque assay. F-actin localized to the periphery of lactotropes. PRL-releasing factors such as TRH, vasoactive intesti...

DFD, when summed up simply, refers to architectural areas in which the structures are either temporary or occasionally timeless. A society, a public space, body of ideas can invest and embed itself structure, is one most important fields architecture since past. Temporary rapidly appear vanish, but they intended do so. Nowadays, there an urgent need for flexibility housing sector. It starts bec...