In vitro immunization of spleen cells from normal BALB/c mice with mitomycin C-treated MOPC-315 tumor cells resulted in high levels of in vitro antitumor cytotoxicity, whereas in vitro immunization of spleen cells from mice bearing large s.c. MOPC-315 tumors resulted in virtually no antitumor cytotoxic ity. The inability of immunized tumor bearer spleen cells to mediate in vitro antitumor cytot...

Seedlings of corn (Zea mays) were tested for recovery from chilling injury incurred at 0.3 +/- 0.3 C. At 0.3 C visual leaf injury appeared in 36 hours, whereas stem and root injuries appeared later. Appearance of leaf injury was preceded by a rise in O(2) uptake and a lessened effect of 2,4-dinitrophenol on O(2) uptake by leaf segments and was accompanied by increased ion leakage from the leave...

1 A technique has been developed for studying over periods of 10 min or longer the effects of drugs on both the force of electrically-induced contractions and the oxygen consumption of an isolated, curarized, mammalian, skeletal muscle preparation.2 The resting oxygen consumption of the muscle was increased substantially by 2,4-dinitrophenol in concentrations (0.02 mM and higher) that eventuall...

The specificity of XapB permease was compared with that of the known nucleoside transporters NupG and NupC. XapB-mediated xanthosine uptake is abolished by 2,4-dinitrophenol and exhibits saturation kinetics with an apparent K(m) of 136 microM. A 12-transmembrane-segment model was confirmed by translational fusions to alkaline phosphatase and the alpha fragment of beta-galactosidase.

Birds have rapidly responding respiratory chemoreceptors [intrapulmonary chemoreceptors (IPC)] that provide vagal sensory feedback about breathing pattern. IPC are exquisitely sensitive to CO(2) but are unaffected by hypoxia. IPC continue to respond to CO(2) during hypoxic and even anoxic conditions, suggesting that they may generate ATP needed for signal transduction anaerobically. To assess I...

A dinitrophenol-based colorimetric chemosensor sequentially sensing Cu2+ and S2?, HDHT ((E)-2-(2-(2-hydroxy-3,5-dinitrobenzylidene)hydrazineyl)-N,N,N-trimethyl-2-oxoethan-1-aminium), was designed synthesized. The selectively detected through a color change of yellow to colorless. calculated detection limit the for 6.4 × 10?2 ?M. In interference test, not considerably inhibited by various metal ...