Dietary proteins elicit a wide range of nutritional and biological functions. Beyond their nutritional role as the source of amino acids for protein synthesis, they are instrumental in the regulation of food intake, glucose and lipid metabolism, blood pressure, bone metabolism and immune function. The interaction of dietary proteins and their products of digestion with the regulatory functions ...

Interaction of human serum albumin with anionic azo dyes decreases the susceptibility of the former to hydrolytic attack by five different proteases. By using methyl orange as the ligand, it is shown that the occupation of the two strong binding sites by the dye is necessary and sufficient for this effect. Different ligands decrease the digestibility of albumin to different extents when tested ...

A common characteristic for proteomic analyses is the need for extensive biochemical processing. Digital microfluidics (DMF), a technique characterized by the manipulation of discrete microdroplets (100 nL-10 microL) on an open array of electrodes, is a good match for carrying out rapid, automated solution-phase reactions. Here, we report a DMF-based method integrating several common processing...

Single stranded DNA (s.s.DNA) comprising 1-2% of the total nuclear DNA was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA3 of embryonic chick cells, labeled for several cell generations with 3H-thymidine. Small quantities of 3H-DNA were annealed with a large excess of unlabeled DNA or polysomal RNA from chick embryos. Hybridization kinetics (monitored by...

Anaerobic digestion is a process that can produce renewable energy through the fermentation of biodegradable biomass. Industrial anaerobic tanks are usually made concrete but production various aggressive compounds (CO2, NH4+ and volatile fatty acids) during microbial leads to deterioration structure. In addition, formation biofilm on cementitious material surface could generate even more inten...