Mouse peritoneal macrophages formed attachments with beta-glucuronidase deficient human fibroblasts within an hour after co-cultures were initiated. Some of these attachments were transitory, while in others macrophages remained in firm contact with fibroblasts for many hours. Attachment of one macrophage did not prevent attachment of others, since many fibroblasts made firm contact with four o...

An iron-controlled infection was obtained after the intraperitoneal infection of Neisseria meningitidis strain M1011 into normal mice. The infection progressed rapidly but then disappeared in concert with the disappearance of plasma transferrin iron. Parenteral iron dextran enhanced and prolonged the infection in mice at dosages above 15 mg of Fe per kg. Studies on the distribution of iron dext...

Salmonella isolates belonging to different serogroups have been analysed with respect to physicochemical surface properties and interaction with human polymorphonuclear granulocytes (PMNs). Most (22/34) recent isolates of the different serotypes showed hydrophilic surface properties and little if any negative charge accompanied by resistance to phagocytosis by PMN similar to the old laboratory ...

The effect of a total dose infusion of iron dextran in pregnancy on 15 mothers and their babies was compared with 19 controls. The bacteriostatic effect and opsonising ability of the sera, of babies born to the treated mothers, were considerably impaired. This was associated with a significantly lower transferrin concentration in these mothers. Although these in vitro tests were not associated ...

We studied the effectiveness with which various charcoal preparations separate free [3H]pteroylglutamate from that complexed with milk folate binder. We tested, in various concentrations, uncoated charcoals and charcoals coated with dextrans of various molecular weights, or with albumin, hemoglubin, or polyvinylpyrrolidone with an average molecular weight of 40 000. Althouth there was some dist...

The dextran sulphates resemble heparin in being active inhibitors of several different forms of biological activity, e.g., blood coagulation, serum complement activity, and certain enzyme systems (Walton, 1954). In each case the activity of the dextran sulphate appears to be dependent upon direct interaction with one or more protein components of the biological system under investigation. In fa...

In a series of pregnant women with iron deficiency anaemia treated by a total dose infusion of iron dextran, the non-haem iron content of the placenta at term was studied histochemically and by chemical analysis. Within a few days of the infusion the Prussian blue reaction on the placenta was very strong, but was negative by ten days after the infusion. Chemical analysis showed that both the wa...

This study deals with the preparation and investigation of a nanoscale delivery system for the anticancer drug doxorubicin (DOX) using its complexation with polyanionic carbohydrate dextran sulfate (DS). Dynamic light scattering, SEM, and zeta potential determination were used to characterize nanocomplexes. DOX-DS complexation was studied in the presence of ethanol as a hydrogen-bond disrupting...

Glucose-grown washed cells of streptococci similar to Streptococcus mutans, which contain cell-bound dextransucrase, have been observed to agglutinate upon the addition of high molecular weight dextran. Low molecular weight dextran or unrelated polysaccharides were ineffective. Agglutination also occurred upon addition of sucrose, which can be converted into dextran, but not with other mono- an...

The slight modification of dextran polysaccharides offers a new family enzyme-responsive emulsifier for encapsulation and controlled release hydrophobic molecules.