Phase separation is a simple, efficient, and cheap method to purify and concentrate detergent-solubilized membrane proteins. In spite of this, phase separation is not widely used or even known among membrane protein scientists, and ready-to-use protocols are available for only relatively few detergent/membrane protein combinations. Here, we summarize the physical and chemical parameters that in...

Dueto theactionofo-quinones formedviapolyphenoloxidase, conserved red clover (Trifolium pratense L.) contains abundant rumen undegradable protein (RUP), but inadequate rumen degradable protein (RDP) fordairy cattle. This studyexaminedhowforagemanagement influences RDP, RUP, crude protein (CP) and fiber fractions in red clover silage and dry matter (DM) yields during the first full production ye...

fonate (CHAPS) is a zwitterionic detergent that was specifically designed for membrane biochemistry. It is known to be a mild, nondenaturing detergent and is a derivative of natural bile salts. Its properties are of interest in several biochemical applications. These include the perturbation of membrane structures, membrane protein solubilization and functional reconstitution, and preparation o...

Detergent surfactants can be found in wastewater in relevant concentrations. Most of them are known as ready degradable under aerobic conditions, as required by European legislation. Far fewer surfactants have been tested so far for biodegradability under anaerobic conditions. The natural environment is predominantly aerobic, but there are some environmental compartments such as river sediments...