The extraction method is critical for the recovery of phenolic compounds. The main goal was to evaluate the effect of an extraction process from mango seed on their phenolic profile, antioxidant and antimicrobial capacities. Phenolic extraction was performed in different steps: maceration, alkaline hydrolysis, acid/alkaline hydrolysis, polar and non-polar fraction of an ethyl acetate separation...

African yam bean (Spenostylis stenocarpa) protein isolate was hydrolyzed with pancreatin (was purchased from sigma chemicals, St Loius Missouri, USA) to obtain an hydrolysate that has (42% degree of hydrolysis), which was characterized for its amino acid composition and ability to scavenge free radicals. The African yam bean protein isolate and hydrolysate samples were dried to constant weight,...

Zygomycetes is a class of fungi living worldwide as saprobes, as part of mycorrhizae, and as parasites. Humans have used some zygomycetes for centuries in the production of traditional foods, e.g. Indonesian tempe. In the present thesis, the experimental focus was on two zygomycetes strains, Mucor indicus CCUG 22424 and Rhizopus sp. IT. One of the distinguishing features of M. indicus is its di...

Itaconic acid is commercially produced by the cultivation of Aspergillus terreus using starch hydrolysate as carbon source. The degree of hydrolysis had a great influence on itaconic acid production which was suitable when corn starch was saccharified at 35 DE. The α-amylase was sufficient to drive the starch hydrolysis to the degree. The agar plate assay with LiCl treatment provided a rapid, s...

Paper mill effluent (PME) was used as an organic feedstock for production of biohydrogen via dark fermentation using heat-shock pretreated anaerobic sludge under mesophilic conditions. The influence of substrate concentration (5, 10 and 15 g-COD/L) and the initial pH (5 and 7) on the efficiency of dark hydrogen fermentation from PME were investigated. The highest hydrogen yield of 55.4 mL/g-COD...

campylobacter jejuni is a major cause of food-borne diarrhea in many countries. poultry and poultry products are known as important sources of human campylobacteriosis. in this study, conventional culture and multiplex pcr methods were compared for the detection of c. jejuni isolated from poultry carcasses. a total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...

Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk concentrate (MPC) reduce immunoreactivity of β-lactoglobulin (β-LG) αs1-casein (αs1-CN). Samples were incubated at an enzyme substrate ratio 1:100 (5.21 units actinidin activity g−1 protein) 10 or 60 °C for up 31 5 h, respectively. Protein hydrolysis determined by the degree SDS-PAGE. Antigenicity hydrolysates β-LG ...