The use of commercially available plastic syringes is introduced as disposable small-scale fixed bed bioreactors for the cultivation of implantable therapeutic cell systems on the basis of an alginate-encapsulated human mesenchymal stem cell line. The system introduced is fitted with a noninvasive oxygen sensor for the continuous monitoring of the cultivation process. Fixed bed bioreactors offe...

Research has been conducted on Spirogyra sp heterotrophic cultivation by providing cassava starch hydrolysate (CSH) as a carbon source under dark condition. The purpose of this research was to obtain the concentration of CSH which could increase biomass concentration and lipid content of Spirogyra sp under heterotrophic cultivation. The treatment of CSH (15 g/L) could produce the highest biomas...

Culture-independent approaches have driven the field of microbiome research and illuminated intricate relationships between the gut microbiota and human health. However, definitively associating phenotypes to specific strains or elucidating physiological interactions is challenging for metagenomic approaches. Recently a number of new approaches to gut microbiota cultivation have emerged through...

The research basically deals with evaluation of the negative impacts of tobacco on health and the environment with a view to minimize tobacco cultivation among the folk peasants in rural Bangladesh. Therefore, the study has been conducted through the survey method with the easy to understand structured questionnaire through which the data are collected by interview and personal observation. How...

Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with ...

BACKGROUND Cell and tissue homeostasis results from the dynamic balance of cell - cell and cell - extracellular component crosstalk that regulates proliferation, differentiation, and apoptosis of cells as well as secretion and activation of soluble factors and/or deposition of extracellular matrix (ECM) components. AIM The aim of the work was to study the crosstalk between tumor cells and str...

background: the main goal of the present study was to set up an axenic cultivation of acanthamoeba and assess the pathogenic ability of t4 genotypes from different clinical and environmental strains of acanthamoeba using two physical assays. methods: sixteen acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. axenic cultivation was performed using ...

The aim of the current study was to investigate if there are any health effects of long-term consumption of organically grown crops using a rat model. Crops were retrieved over two years from a long-term field trial at three different locations in Denmark, using three different cultivation systems (OA, organic based on livestock manure; OB, organic based on green manure; and C, conventional wit...

In this study, the effects of temperature-shift (from 30 to 25 °C) and temperature-constant (at 30 °C) cultivation on the mass of Monascus fuliginosus CG-6 mycelia and concentration of the produced monacolin K (MK) were monitored. The expression levels of the MK biosynthetic genes of M. fuliginosus CG-6 at constant and variable culture temperatures were analysed by real-time quantitative polyme...

Shifting cultivation in the humid tropics is incredibly diverse, yet research tends to focus on one type: long-fallow shifting cultivation. While it is a typical adaptation to the highly-weathered nutrient-poor soils of the Amazonian terra firme, fertile environments in the region offer opportunities for agricultural intensification. We hypothesized that Amazonian people have developed divergen...