Antibodies which fail to precipitate with the monomeric homologous cytochromes c were elicited in rabbits by the conjugate of bovine y-globulin and cytochrome c (tuna and turkey proteins) or by free monomeric cytochrome c (kangaroo and, in one case, the horse protein). Such antibodies do form precipitates and fix complement when allowed to react with the polymerized homologous antigen. In contr...

The nitrated polycyclic aromatic hydrocarbon 1-nitropyrene is a ubiq uitous environmental pollutant. The role of cytochromes P-450 in the human metabolism of [3II]-l-nitropyrene was investigated using human liver microsomes. The range of microsomal metabolism from 16 indi vidual liver specimens was 0.13 to 0.99 nmol/min/mg protein. Using 3 microsomal samples exhibiting different maximal velocit...

Two electrophoretically homogeneous forms of liver microsomal cytochrome P-450 (P-~~OLM), designated P-450~~~ and LM~ by their relative mobilities, were previously isolated from rabbits induced with phenobarbital and P-naphthoflavone, respectively. In the present immunochemical study, rabbits and goats were inoculated with these purified proteins. Rabbits produced antibodies against P-450~~~ bu...

BACKGROUND & AIMS The liver has one of the highest rates of heme synthesis of any organ. More than 50% of the heme synthesized in the liver is used for synthesis of P450 enzymes, which metabolize exogenous and endogenous compounds that include natural products, hormones, drugs, and carcinogens. Feline leukemia virus subgroup C cellular receptor 1a (FLVCR1a) is plasma membrane heme exporter that...

Previous studies demonstrated that an outer membrane c-type cytochrome, OmcB, was involved in Fe(III) reduction in Geobacter sulfurreducens. An OmcB-deficient mutant was greatly impaired in its ability to reduce both soluble and insoluble Fe(III). Reintroducing omcB restored the capacity for Fe(III) reduction at a level proportional to the level of OmcB production. Here, we report that the OmcB...

The electrochemical midpoint potentials (E(m)'s) of 13 cytochromes, in globin (c, c(2), c(551), c(553)), four-helix bundle (c', b(562)), alpha beta roll (b(5)), and beta sandwich (f) motifs, with E(m)'s spanning 450 mV were calculated with multiconformation continuum electrostatics (MCCE). MCCE calculates changes in oxidation free energy when a heme-axial ligand complex is moved from water into...

The nitrated polycyclic aromatic hydrocarbon 1-nitropyrene is a ubiquitous environmental pollutant. The role of cytochromes P-450 in the human metabolism of [3H]-1-nitropyrene was investigated using human liver microsomes. The range of microsomal metabolism from 16 individual liver specimens was 0.13 to 0.99 nmol/min/mg protein. Using 3 microsomal samples exhibiting different maximal velocities...

Two electrophoretically homogeneous forms of liver microsomal cytochrome P-450 (P-~~OLM), designated P-450~~~ and LM~ by their relative mobilities, were previously isolated from rabbits induced with phenobarbital and P-naphthoflavone, respectively. In the present immunochemical study, rabbits and goats were inoculated with these purified proteins. Rabbits produced antibodies against P-450~~~ bu...