The bearing of lipid solubilities and substantivities of dyes on the results of cytochemical staining reactions is briefly discussed. Solubilities of indigoid dyes in various lipids are qualitatively similar to those in symtetrachloroethane. Quantitative determinations made in this solvent indicate that, in general, indigo and its 5:5'and 6:6'-substituted derivatives are sparingly soluble, wher...

Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7...

The fine structure and cytochemistry of the extremely large RNA puffs, or Balbiani rings, in salivary gland nuclei of midge, Chironomus thummi, larvae have been investigated. The Balbiani rings are composed of a diffuse mass of electron-opaque 400 to 500 A granules, short threads about 180 to 220 A in diameter and associated fine chromatin fibrils. These components appear to be organized into b...

PURPOSE To evaluate somatic mitochondrial (mt)DNA mutations in the macula during ageing. METHODS Ten 30-microm cryostat sections from the macula (foveal and perifoveal regions) and peripheral retina of 14 donors (aged 14-94 years) were cut for cytochrome c oxidase cytochemistry. The photoreceptor layer was microdissected and DNA extracted for 4977-bp mtDNA (mtDNA(4977)) quantification using P...

This study was carried out at the department of Oral Pathology, Queen Mary College of Medicine and Dentistry, Barts and The London to determine whether human oral buccal mucosal (keratinocytes) cell line, TR 146, expressed iNOS message and whether the expression of iNOS is varied when TR146 cells were exposed to different cytokines such as IL-15, IL-8, IL-1 β, or TNFα using RT-PCR and Immuno-cy...