BACKGROUND Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1beta (IL1beta), using the cell wall protein Pir4 as fusion partner. To achieve high cell density and, consequently, high product yield, BY4741 [PIR4-IL1beta] was cultured in an aerated fed-batch reactor, using a defined m...

Bacterial extracellular electron transfer (EET) is envisioned for use in applied biotechnologies, necessitating electrochemical characterization of natural and engineered electroactive biofilms under conditions similar to the target application, including small-scale biosensing or biosynthesis platforms, which often distinct from standard 100 mL-scale stirred-batch bioelectrochemical test platf...

The aim of this article is to present the Iterative Model Predictive Controller, inmpc, as a good candidate to control chemical batch reactors. The proposed control approach is derived from a model-based predictive control formulation which takes advantage of the repetitive nature of batch processes. The proposed controller combines the good qualities of Model Predictive Control (mpc) with the ...

Sequencing batch reactor (SBR) is one of several periodically operated, suspended-growth activated sludge system. It has an advantage for nitrogen removal which is difficult to remove with conventional system. This paper reviewed the nitrogen removal performance in SBR for several different industrial wastewaters and discussed the factors which may affect the treatment performance. Several modi...

Microbial electrosynthesis (MES) is a potential technology for CO2 recycling, but insufficient information available on the microbial interactions underpinning electrochemically-assisted reactions. In this study, MES reactor was operated 225 days alternately with bicarbonate or as carbon source, under batch continuous feeding regimens, to evaluate response of communities, and their productivity...

the performance of a plug flow electrocoagulation reactor for the treatment of cattle abattoir(slaughterhouse) wastewater was investigated. a cylindrical iron reactor was operated as a cathode at arecycling batch mode while three iron rods located at the center of the reactor were used as an anode. theeffects of different parameters, including the current density, the supporting electrolyte dos...

This study presents an investigation of electrochemical variables and an application of the optimal parameters in operating a continuous upflow electrocoagulation reactor in removing dye. Direct red 23, which is azo-based, was used as a representative of direct dyes. First, a batch mode was employed to optimize the design parameters: electrode type, electrode distance, current density and elect...

Fermentation of sugar by Saccharomyces cerevisiae, for production of ethanol in an immobilized cell reactor (ICR) was successfully carried out to improve the performance of the fermentation process. The fermentation set-up was comprised of a column packed with beads of immobilized cells. The immobilization of S. cerevisiae was simply performed by the enriched cells cultured media harvested at e...

This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the support (adsorbent) or form flocs/aggregates (also called granules) without use of chemicals and form thick layers of cells known as "biofilms." As a result of biofilm formation, cell densi...

In the chemical industry, final products arise from chemical and physical transformations of materials in processing units. We consider the case of batch production mode, where the total requirements for intermediate and final products are divided into individual batches. To produce a batch, the inputs are first loaded into a processing unit, then a transformation process is executed, and final...