D-type cyclins (cyclins D1, D2, and D3) are key components of cell cycle machinery in mammalian cells. These proteins are believed to drive cell cycle progression by associating with their kinase partners, cyclin-dependent kinases, and by directing phosphorylation of critical cellular substrates. In addition, D-cyclins play a kinase-independent role by sequestering cell cycle inhibitors p27(Kip...

Cyclin D1 is a multifunctional protein that activates CDK4 and CDK6, titrates Cip/Kip CDK inhibitors to increase CDK2 activity, and modulates the function of certain transcription factors. To specifically test the importance of cyclin D1-associated kinase activity, we generated "knockin" mice expressing mutant cyclin D1 deficient in activating CDK4/6. The development of several cyclin D1-depend...

D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene (Ccnd1) have been deleted and replaced by those of human cyclin E (CCNE). In the tissues and cells of these mice, the expression pattern of human cyclin E faithfully reproduces that normally associated with mouse cyclin D1....

lates with the maturational stage of these cells. We have studied cyclin D1 expression in the inner ear. Besides its well known function in the cell cycle control, cyclin D1 has also been implicated in other cellular processes like cell death, cellular differentiation and cancer. During early embryogenesis cyclin D1 is widely expressed in the developing sensory epithelia, but it becomes downreg...

Enantiomerically pure (S)-tributylstannyl[D1]methanol and (R)- and (S)-tributylstannyl[D1]methyl benzoates were Stille-coupled with bromobenzene and benzoyl chloride in 1,4-dioxane and toluene using [(Ph3P)4Pd] or [(Ph3P)2PdCl2] either alone or in combination with CuCN as cocatalyst at temperatures up to 80 °C. The products were found to be enantiomerically pure. (R)- and (S)-N-(tributylstannyl...

lates with the maturational stage of these cells. We have studied cyclin D1 expression in the inner ear. Besides its well known function in the cell cycle control, cyclin D1 has also been implicated in other cellular processes like cell death, cellular differentiation and cancer. During early embryogenesis cyclin D1 is widely expressed in the developing sensory epithelia, but it becomes downreg...

Both cyclin D1 and estrogens have an essential role in regulating proliferation of breast epithelial cells. We show here a novel role for cyclin D1 in growth regulation of estrogen-responsive tissues by potentiating transcription of estrogen receptor-regulated genes. Cyclin D1 mediates this activation independent of complex formation to a CDK partner. Cyclin D1 activates estrogen receptor-media...

The major physiologic function of type 1 iodothyronine deiodinase (D1) is to produce triiodothyronine (T(3)) for the plasma. D1 activity is regulated by numerous factors, perhaps the most important of which in human pathophysiology is T(3). T(3) induces D1 expression, contributing to the T(3) excess commonly found in hyperthyroidism. Cytokines, nutritional status, sex steroids, and other factor...

Aging is associated with an increase in oxidative stress. Previously, we have reported that dopamine failed to inhibit proximal tubular Na-K-ATPase and to promote sodium excretion in old rats (Beheray S, Kansra V, Hussain T, and Lokhandwala MF. Kidney Int 58: 712-720, 2000). This was due to uncoupling of dopamine D1 receptors from G proteins resulting from hyperphosphorylation of D1 receptors. ...