background and objectives: diagnosis of avian tuberculosis by conventional culture method is still considered as the “gold standard” technique. the main objective of this study was to compare growth of mycobacterium avium subsp. avium on four specific mycobacterial cultures such as glycerinated lowenstein-jensen medium, pyruvate-enriched lowenstein- jensen medium, mycobactin j-supplemented herr...

the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

This study evaluated the effects of superoxide dismutase (SOD) on viability and acrosome integrity of European mouflon spermatozoa after cryopreservation and on the fertilization rates of sheep oocytes after i.v.f. or intracytoplasmatic sperm injection (i.c.s.i.). Frozen semen was thawed and washed with synthetic oviduct fluid supplemented with 0.6% bovine serum albumin. After centrifugation, t...

The green colonial microalga, Botryococcus braunii is well known for its high lipid content and has already been proposed as a renewable energy source for various aquaculture and biotechnological applications. However, due to its slow growth rate compared with other microalgae, B. braunii has not yet been used in mass culture to produce more biomass. Therefore, in this study we tested different...

background: atrazine is a widely used herbicide. the increasing salinity of many water resources has had a negative effect on atrazine biodegradation. the aim of this study was to isolate atrazine degrading bacteria in semi-salinity media.   methods: nine selected bacterial species were cultivated on the mineral salt broth culture medium containing atrazine (50, 100, 500 mg/l), nacl concentrati...

introduction: both initiation and maintenance of spermatogenesis are hormonally regulated by fsh and testosterone. in this study, the effects of fsh and testosterone on the in vitro maturation of fresh and frozen- thawed round spermatids were determind. material and methods: cell suspension was isolated from testis of nmri male mice (8-10 weaks old) and divided into two groups .the first part w...

The green colonial microalga, Botryococcus braunii is well known for its high lipid content and has already been proposed as a renewable energy source for various aquaculture and biotechnological applications. However, due to its slow growth rate compared with other microalgae, B. braunii has not yet been used in mass culture to produce more biomass. Therefore, in this study we tested different...

MATSUYA, Y. and YAMANE, I. Culture Conditions Necessary to Sustain the Serial Culture of Lung Cells of Newborn Hamster in Albumin Fortified Medium. Tohoku J. exp. Med., 1970, 101(4), 387-396•\Using various albumin fortified medium, many neonatal hamster lung cells were developed into established lines. The optimal culture condition to evolve into cell lines was investigated by varying the sourc...

Background: There is a considerable numbers of buffalo (Bubalus bubalis) in Iran which have an important role in rural life. The reproductive performance of buffalo is lower than cow. Co-culture with somatice cells improved the maturation rate of most of the species. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on post fertilization development of buffalo...

tolerance of two apple rootstocks (gami and m26) was evaluated at different concentrations of sodium chloride in vitro conditions. micro plants grown under ms medium culture were transferred to knoudson–c culture medium devoid of na and cl ions. concentrations of nacl in the knoudson-c culture medium were 0, 20, 40, 60, 80 and 100 mm/l. six weeks after culturing, data were collected. the result...