Chalcone synthase (CHS) is a plant-specific enzyme that synthesises naringenin chalcone, an essential precursor of the flavonoid biosynthetic pathway. Naringenin and kaempferol are two flavonoids that have been demonstrated to inhibit the proliferation of HeLa cells. To study chalcone synthase gene regulation in Melastoma decemfidum, we developed a high-yield total RNA isolation method to assem...

Equilibrium unilamellar vesicles are stabilized by one of two distinct mechanisms depending on the value of the bending constant. Helfrich undulations ensure that the interbilayer potential is always repulsive when the bending constant, K, is of order k(B)T. When K k(B)T, unilamellar vesicles are stabilized by the spontaneous curvature that picks out a particular vesicle radius; other radii are...

Dermanyssus gallinae is one of the most serious ectoparasites of poultry and it has been implicated as a vector of several major pathogenic diseases. Molecular detection of such pathogens in mites is crucial and therefore, an important step is the extraction of their DNA from mites. So, we compared four DNA extraction protocols from engorged and unfed individual mites: a conventional method usi...

Brucellosis and leptospirosis are zoonotic diseases and cause much economic losses in livestock by causing abortion, stillbirth and infertility. Both organisms are excreted in milk and thus increasing the risk of infecting other animals and human. Early detection of organisms is desirable for effective treatment and control of diseases. In this study, multiplex PCR was attempted to detect Bruce...

Our study includes removal of Cr(VI) by using mixed surfactants (Non-ionic: Cationic). A common cationic surfactant Cetyl trimethyl ammonium bromide(CTAB) with two different nonionic surfactant Triton X-100 and Tween-80 were used to remove Cr(VI) by micellar enhanced ultrafiltration technique.The effect of parameters such as initial feed metal ion concentration, pH and filtration time on % Reje...

Mesoporous silica materials with a variety of morphologies, such as monodisperse microspheres, gigantic hollow structures comprising a thin shell with a hole, and gigantic hollow structures consisting of an outer thin shell and an inner layer composed of many small spheres, have been readily synthesized in mixed water-ethanol solvents at room temperature using cetyltrimethylammonium bromide (CT...

The physical properties of ionic surfactants are very sensitive to the nature of the counterions. In particular, counterions that have a tendency to adsorb on the surfactant micelle are known to dramatically modify the viscoelastic properties of these solutions through the formation of long wormlike micelles [1, 2, 3, 4]. One extreme limit of an adsorbed counterion is an oppositely charged surf...

It was shown by the conductometric study that formation of micelles in cetyltrimethylam¬monium bromide (CTAB) – dimethylsulfoxide (DMSO) water system occurs at higher critical micelle concentrations (CMC) than CTAB system. The solubilization nitroxoline this upon reaching CMC determined spectrophotometric method. Box–Behnken design used to obtain systems with highest light absorption nitroxolin...

Mesoporous silica nanomaterials with varied morphologies and pore structures, including nanospheres, nanoellipsoids, helical nanorods and multi-lamellar nanovesicles, were synthesized by using cetyltrimethylammonium bromide (CTAB) and sodium bis(2-ethylhexyl) sulfosuccinate (AOT) as co-templates.