9,10-Dihydro-9,10-ethano-anthracene-11,12-dicarboxylic acid disodium salt (DHEAS) was synthesized and used as a nucleating agent for poly(1-butene) (iPB). The isothermal crystallization kinetics of iPB having different nucleating agents were investigated by differential scanning calorimetry (DSC) and polarized optical microscopy (POM). The results showed that the nucleating agents increased the...

Co-crystallization experiments of proteins with nucleic acids do not guarantee that both components are present in the crystal. We have previously developed DIBER to predict crystal content when protein and DNA are present in the crystallization mix. Here, we present RIBER, which should be used when protein and RNA are in the crystallization drop. The combined RIBER/DIBER suite builds on machin...

In this paper, a simple low-cost alternative to large commercial systems for preparing macromolecular crystallization conditions is described. Using an intuitive spreadsheet-based approach, the system allows the rapid calculation of relevant pipetting volumes given known stock-solution concentrations and incorporates the automatic design of custom crystallization screens via the incomplete-fact...

The amorphous to microcrystalline phase transition of Ge in Pb/Ge multilayers has been extensively studied. During crystallization, the x-ray diffraction peaks of the modulated structure disappear and the Pb texture improves. It is shown that the crystallization temperature decreases with decreasing amorphous Ge thickness and is strongly affected by the texture of the metallic component. These ...

Why are proteins so hard to crystallize? We propose an 'evolutionary negative design' principle to explain this difficulty. Proteins have evolved to avoid crystallization because crystallization compromises the viability of the cell. Evolutionary negative design is supported by much evidence in the literature, including the effect of mutations on the crystallizability of a protein, the correlat...

Using Monte Carlo simulations we study crystallization in the three-dimensional Ising model with four-spin interaction. We monitor the morphology of crystals which grow after placing crystallization seeds in a supercooled liquid. Defects in such crystals constitute an intricate and very stable network that separates various domains by tensionless domain walls. We also show that the crystallizat...

PURPOSE Gokhsuradi churna is an ayurvedic formulation, was investigate for antiurolithiatic activity. METHODS Calcium oxalate crystallization was induced by the addition of 0.01M sodium oxalate solutions in synthetic urine and nucleation method. RESULTS The effect of Gokhsuradi Churna exhibited a concentration dependent inhibition of on calcium oxalate crystallization and nucleation. CONC...

BACKGROUND Rasburicase (Fasturtec® or Elitek®, Sanofi-Aventis), the recombinant form of urate oxidase from Aspergillus flavus, is a therapeutic enzyme used to prevent or decrease the high levels of uric acid in blood that can occur as a result of chemotherapy. It is produced by Sanofi-Aventis and currently purified via several standard steps of chromatography. This work explores the feasibility...

BACKGROUND Protein crystallization is a slow process of trial and error and limits the amount of solved protein structures. Search of a universal heterogeneous nucleant is an effort to facilitate crystallizability of proteins. METHODOLOGY The effect of polystyrene nanospheres on protein crystallization were tested with three commercial proteins: lysozyme, xylanase, xylose isomerase, and with ...

In this work, a series of polypropylene/polyvinyl butyral (PP/PVB) blends were prepared by melt-blending process, at PVB loadings 3 wt%, 10 wt%, and 30 wt%. The effects of PVB on crystallization behavior of PP were investigated by differential scanning calorimetry (DSC), and polarized optical microscopy (POM). The isothermal crystallization kinetics were analyzed by Avrami equations. It was fou...