This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study.  The hatching rates of embryos exp...

Vitrification is widely used for cryopreservation of oocytes. The present study has built the theoretical models of four vitrification systems (micro-droplet, open pulled straws, quartz micro-capillary and cryotop), and performed numerical analysis to predict the cooling rates. The numerical analysis shows that the average cooling rate of the cryotop system was higher than those of other three ...

The first step of organ cryopreservation is a cryoprotectant perfusion, which can speed cooling. The temperature of the kidney decreased from 37°C to about 0°C by perfusion. During the kidney perfusion process, the cryoprotectant enters the kidneys through the renal artery and leaves the kidneys through the renal vein after passing through a series of capillaries. In cold perfusion vessels with...

Different factors may affect the sensitivity of porcine oocytes during cryopreservation. The effect of two methods (cooling and vitrification), four cryoprotectants [glycerol (GLY), 1, 2-propanediol (PROH), dimethyl sulfoxide (DMSO) or ethylene glycol (EG)] and two vitrification media (1 M sucrose (SUC)+8 M EG; 8 M EG) on the developmental capacity of porcine oocytes at the germinal vesicle (GV...

Cryonics technology seeks to cryopreserve the anatomical basis of the mind so that future medicine can restore legally dead cryonics patients to life, youth, and health. Most cryonics patients experience varying degrees of ischemia and reperfusion injury. Neurons can survive ischemia and reperfusion injury more than is generally believed, but blood vessels are more vulnerable, and such injury c...

The principle of sperm preservation is to prolong the viability spermatozoa by reducing degree metabolism during storage at low temperatures, and one method involved was a cryoprotectant as protector. This study aimed determine best type for refrigeration goldfish Carrasius auratus sperm. A completely randomized design used, five types were tested, namely Methanol, Ethanol, Ethylene Glycol, Gly...