Cryo-thermal therapy has been emerged as a promising novel therapeutic strategy for advanced breast cancer, triggering higher incidence of tumor regression and enhanced remission of metastasis than routine treatments. To better understand its anti-tumor mechanism, we utilized a spontaneous metastatic mouse model and quantitative proteomics to compare N-glycoproteome changes in 94 serum samples ...

We present CryoGAN, a new paradigm for single-particle cryo-electron microscopy (cryo-EM) reconstruction based on unsupervised deep adversarial learning. In cryo-EM, the structure of biomolecule needs to be reconstructed from large set noisy tomographic projections with unknown orientations. Current techniques are marginalized maximum-likelihood formulation that requires calculations over all p...

The effects of ultrasonic impact treatment (UIT) and UIT with equal channel angular pressing (ECAP), on the microstructure Cu37Zn alloy are studied at ambient cryogenic temperatures. With decreasing process temperature, highly twinned areas submicro/nano-scale thickness/spacing, together dislocation tangles/cells, introduced in alloy. Direct microscopy observation stored energy release analysis...

Synthetic multichromophore systems are of great importance in artificial light harvesting devices, organic optoelectronics, tumor imaging and therapy. Here, we introduce a promising strategy for the construction self-assembled peptide templated dye stacks based on coupling de novo designed pH sensitive with cyanine Cy5 at its N-terminus. Microscopic techniques, particular cryogenic TEM (cryo-TE...

Abstract Different tasks in the computational pipeline of single-particle cryo-electron microscopy (cryo-EM) require enhancing quality highly noisy raw images. To this end, we develop an efficient algorithm for signal enhancement cryo-EM The enhanced images can be used a variety downstream tasks, such as two-dimensional classification, removing uninformative images, constructing ab initio model...

ConspectusElectron microscopy (EM) is the most versatile tool for study of matter at scales ranging from subatomic to visible. The high vacuum environment and charged irradiation require careful stabilization many specimens interest. Biological samples are particularly sensitive due their composition light elements suspended in an aqueous medium. Early investigators developed techniques embeddi...

Focused Ion Beam-Induced Deposition (FIBID) is a single-step nanopatterning technique that applies focused beam of ions to induce the decomposition gaseous precursor. The processing rate FIBID increases by two orders magnitude when process performed at cryogenic temperatures (Cryo-FIBID): precursor forms condensed layer on surface cooled substrate, greatly enhancing amount material available fo...

Advances in cryo-EM technology will open a new era of RNA-only 3D structure determination.