Antibodies raised against recombinant Loxosceles intermedia dermonecrotic protein isoform 1 (rLiD1) display neutralizing capacity for the L. intermedia whole venom. We previously found that an immunodominant continuous B-cell epitope, recognized by these antibodies corresponds to a region of the protein known to be involved in the active site. In this study, we extend previous work by preparing...

After being envenomated by the timber rattlesnake, a patient was found to have a platelet count of 5000 per microliter, prothrombin time and activated partial thromboplastin time both greater than 150 sec, plasma fibrinogen 0 mg/dl, and fibrinogen split products 2560 microgram/ml. However, this patient did not appear to have acute disseminated intravascular coagulation since coagulation factors...

Cobra venoms contain heat stable, basic, proteins that inhibit iodide accumulation by thyroid slices. Activity is not related to the content of phospholipase A or various other enzymatic activities. These proteins are present only in Elapid venoms and not in snakes of the Crotalidae or Viperidae families. Two such basic proteins, named cobramines A and B, have been purified from the crude venom...

Early studies in the 1930s on the venom of South American Lancehead snakesofthe Bothrops genuslead to the discovery of compounds active in blood coagulation such as batroxobin and botrocetin. The scope of our investigations is to have a deeper look at the crude venom of B. moojeni using state-of-the-art proteomics methods, as well as newly developed bioassays screening for activities in the dif...

Enzymes that catalyze the oxidation of amino acids have been found in microorganisms, mammals, and lower animals. As yet, few of these have been obtained in a state of high purity, and many studies on these enzymes have therefore been carried out with relatively crude preparations. The detailed mechanism of the flavoprotein-cat.alyzed oxidative dcamination of amino acids remains to be <Jlucidat...

Bothrops asper (Squamata: Viperidae) is the most important venomous snake in Central America, being responsible for the majority of snakebite accidents. Four basic PLA2s (pMTX-I to -IV) were purified from crude venom by a single-step chromatography using a CM-Sepharose ion-exchange column (1.5 × 15 cm). Analysis of the N-terminal sequence demonstrated that pMTX-I and III belong to the catalytic...

After being envenomated by the timber rattlesnake. a patient was found to have a platelet count of 5000 per tl. prothrombin time and activated partial thromboplastin time both greater than 1 50 sec. plasma fibrinogen 0 mg/dl. and fibrinogen split products 2560 sg/ml. However. this patient did not appear to have acute disseminated intravascular coagulation since coagulation factors II-XIl were n...

After being envenomated by the timber rattlesnake. a patient was found to have a platelet count of 5000 per tl. prothrombin time and activated partial thromboplastin time both greater than 1 50 sec. plasma fibrinogen 0 mg/dl. and fibrinogen split products 2560 sg/ml. However. this patient did not appear to have acute disseminated intravascular coagulation since coagulation factors II-XIl were n...

Technetium-99m (Tc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, Tc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The...

Cobra venoms contain heat stable, basic, proteins that inhibit iodide accumulation by thyroid slices. Activity is not related to the content of phospholipase A or various other enzymatic activities. These proteins are present only in Elapid venoms and not in snakes of the Crotalidae or Viperidae families. Two such basic proteins, named cobramines A and B, have been purified from the crude venom...