The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were aspartic and glutamic acid (minor ones) as well as glycine, alanine, and valine (...

test were retested over a suitable range of concen trations to determine their degree of activity. Third, in vitro tests were performed with bacteria free crown-gall tissues and healthy tissues to de termine whether differences existed in the sensi tivity of these tissues to the growth inhibitors. Fourth, where growth inhibition was considered to be due to the displacement of an essential metab...

The expression of cauliflower mosaic virus proteins was examined in crown gall tissue transformed by integration of virus genome DNA into plant host chromosomes by Agrobacterium tumefaciens-mediated transfer. Galls upon plants of species that support infection by virus particles as well as upon non-host plants were analysed by immunoblotting using antisera specific to proteins encoded by viral ...

gene can be spliced to the virus vector. Also, the added gene affects the cell-to-cell movement (invasiveness) of the virus, since the virus can no longer mature. Moreover, the virus is mainly limited to Cruciferae as host plants and therefore the prospects of infecting other major crops are narrow. Nevertheless, in-depth studies on this virus will help provide important information on means to...

The cytokinin complex of Datura innoxia Mill. crown gall tissue was purified by ion exchange, Sephadex LH-20 chromatography and reversed-phase high performance liquid chromatography. By gas chromatography-mass spectrometry using (2)H-labeled compounds, the following cytokinins were identified in the basic fraction eluting from a cation exchange column: zeatin, zeatin riboside, dihydrozeatin, di...

Several bacteria were evaluated in greenhouse studies for their effectiveness at preventing formation of galls induced by Rhizobium vitis, the Gram negative bacterium that causes crown gall disease of grapevines. Three endophytic bacteria isolated from Malus domestica yielded promising results and were selected for further examination: Pseudomonas fluorescens isolate 1100-6, Bacillus subtilis i...

Two novel oxazolomycin isomers, oxazolomycins B (2) and C (3), were isolated from the fermentation broth of an oxazolomycin-producing strain, Streptomyces albus JA3453. Both compounds are geometrical isomers of oxazolomycin (1), the configurations of their triene moieties being (4'E, 6'E, 8'E) (2) and (4'Z, 6'E, 8'E) (3) while that of oxazolomycin (1) is (4'Z, 6'Z, 8'E). Compounds 2 and 3 exhib...

Crops can be devastated by pathogenic strains of Agrobacterium tumefaciens that cause crown gall tumors. This devastation can be prevented by the nonpathogenic biocontrol agent A. radiobacter K84, which prevents disease by production of the "Trojan horse" toxin agrocin 84, which is specifically imported into tumorgenic A. tumefaciens strains to cause cell death. We demonstrate that this biocont...

11. STEWARD, F. C. AND S. M. CAPLIN. 1954. The growth of carrot tissue explants and its relation to the growth factors present in coconut milk. I. (A) The development of the quantitative method and the factors affecting the growth of carrot tissue explants. Ann. Biol. 30: 385-94. 12. TAMAOKI, T., A. C. HILDEBRANDT, R. H. BURRIS, AND A. J. RIKER. 1961. Oxidation of reduced diphosphopyridine nucl...

Attempts were made to provide proof for the occurrence of cyclic 3',5'-adenosine monophosphate in healthy and crown gall tissues of Vicia faba. Although our purified extracts gave positive readings in the Gilman binding assay for cyclic AMP, they were not digested by a specific cyclic 3',5'-adenosine monophosphate phosphodiesterase from beef heart. The extracts were digested, however, by a part...