Case report A 66-year-old woman presented with rapidly progressive chest pain. Her medical history was positive for a long-standing diabetes mellitus type 2, hypertension, and dyslipidemia. Her initial workup showed diffuse ST-segment depressions on a 12-lead ECG, and positive biomarkers including troponin I (Tn-I) 4.2 ng/ml (upper normal limit (UNL) 0–0.15 ng/ml), creatine kinase (CK) 254 U/l ...

BACKGROUND The combination of bicarbonate and mannitol (BIC/MAN) is commonly used to prevent renal failure (RF) in patients with rhabdomyolysis despite the absence of sufficient evidence validating its use. The purpose of this study was to determine whether BIC/ MAN is effective in preventing RF in patients with rhabdomyolysis caused by trauma. METHODS This study was a review of all adult tra...

Chemical modification of rabbit muscle creatine kinase (CK) with thiol-specific reagents led to partial or complete inactivation of the enzyme. Using site-directed mutagenesis, we have substituted the corresponding reactive Cys278 in the chicken cardiac mitochondrial creatine kinase (Mib-CK) with either glycine, serine, alanine, asparagine, or aspartate. The resulting mutant Mib-CK enzymes show...

Three surveys of the measurement and interpretation of creatine kinase (CK; EC 2.7.3.2) isoenzyme 2 (CK-MB) were conducted in Ontario, Canada, in 1989. Of the clinical laboratories participating, 66% used immunological methods and 24% used electrophoretic methods. Although reference ranges and interpretative routines varied widely, 95% of the laboratories reported correct interpretations for 10...

Fukuyama congenital muscular dystrophy (FCMD) is the second most prevalent childhood-onset in Japan. It an autosomal recessive disorder caused by fukutin mutation (FKTN), characterized muscle wasting and brain abnormalities. So far, serum creatine kinase (CK) recognized as only biomarker for FCMD. Recently, ELISA assay to quantify N-terminal fragment of titin urine was developed. Urinary concen...

Objective: Coronavirus-2019 (COVID-19) was declared a pandemic by the World Health Organization, continues to spread globally, and patients may not exhibit specific musculoskeletal involvement symptoms. Materials Methods: Clinical data images of 389 consecutive patients, who applied outpatient clinic with COVID-19 pre-diagnosis complaints, 203 control underwent thoracic computed tomography (CT)...

We compared three current methods (immunoinhibition, "Isomune-CK" immunoprecipitation, and the Tandem-E CKMB II immunoenzymometric assay) for determination of creatine kinase (CK; EC 2.7.3.2) isoenzyme MB in serum. Although results inter-correlated well, the immunoinhibition assay gave higher activity values. Atypical CK forms did not interfere with the immunoprecipitation and immunoenzymometri...

I measured total creatine kinase (CK; EC 2.7.3.2) activity and isoenzyme pattern in normal and neoplastic tissues. CK activity was detected in all of them examined. In various tumors it was greater than, less than, or the same as that in normal tissue, no clear correlation being seen between total activity and growth rate or degree of differentiation. In several cases, there was a greater propo...

Creatine kinase isoenzyme I(BB) is generally not detectable in normal serum, and its occurrence in serum has been documented in only a few disease states. In particular, increased activity of this isoenzyme has been reported in association with chronic renal failure, hemodialysis, and renal transplantation. The present study demonstrates that the apparent creatine kinase observed in the serum o...