PURPOSE To evaluate the optical aberrations induced by LASIK refractive surgery for myopia on the anterior surface of the cornea and the entire optical system of the eye. METHODS Total and corneal aberrations were measured in a group of 14 eyes (preoperative myopia ranging from -2.5 to -13 D) before and after LASIK surgery. Total aberrations were measured using a laser ray-tracing technique. ...

PURPOSE To investigate the test-retest variability of and factors associated with the corneal deformation response, and evaluate its impact on IOP measurement. METHODS Forty normal and 140 glaucoma suspect/glaucomatous eyes of 104 individuals were prospectively included for repeated measurements of corneal deformation response by an ultra-high-speed Scheimpflug camera, followed by corneal hys...

Key issues in corneal epithelium biology are the mechanism for corneal epithelium stem cells to maintain the corneal epithelial homeostasis and wound healing responses, and what are the regulatory molecular pathways involved. There are apparent discrepancies about the locations of the progenitor populations responsible for corneal epithelial self-renewal. We have developed a genetic mouse model...

PURPOSE To evaluate the performance of corneal topographic astigmatism (CorT) based on total corneal power measurements. METHODS Anterior, posterior, and total corneal power measurements of 526 virgin eyes obtained using the CSO Sirius tomographer (Costuzione Strumenti Oftalmici, Scandicci, Florence, Italy) were analyzed. Individual CorTs were created from each set of data. These CorTs were a...

PURPOSE To investigate corneal abnormalities in heterozygous Pax6(+/Sey-Neu) (Pax6(+/-), small eye) mice and compare them with aniridia-related keratopathy in PAX6(+/-) patients. METHODS Fetal and postnatal corneal histopathology, adult corneal thickness, and the distribution of K12-immunostained cells were compared in wild-type and Pax6(+/-) mice. RESULTS Prenatally, the corneal epithelium...

Substance P (SP) is a neuropeptide, predominantly released from sensory nerve fibers, with a potentially protective role in diabetic corneal epithelial wound healing. However, the molecular mechanism remains unclear. We investigated the protective mechanism of SP against hyperglycemia-induced corneal epithelial wound healing defects, using type 1 diabetic mice and high glucose-treated corneal e...

Substance P (SP) is a neuropeptide, predominantly released from sensory nerve fibers, with a potentially protective role in diabetic corneal epithelial wound healing. However, the molecular mechanism remains unclear. We investigated the protective mechanism of SP against hyperglycemia-induced corneal epithelial wound healing defects, using type 1 diabetic mice and high glucose-treated corneal e...

purpose: to assess alterations in parameters measured by the ocular response analyzer (ora) after photorefractive keratectomy (prk) with or without 0.02% mitomycin c (mmc) in myopic eyes. methods: in 33 right eyes of 33 patients, iop was determined using the goldmann applanation tonometry (gat) before and 3 months after prk. corneal hysteresis (ch), corneal resistance factor (crf), goldmann-cor...

purpose: to describe a novel surgical technique for in vivo limbal stem cell cultivation for reconstruction of corneal epithelium in total limbal stem cell deficiency (lscd). methods: patients suffering from chronic total unilateral lscd due to a chemical injury were sequentially included. one small limbal block (2x1 mm) harvested from contralateral healthy eye sutured over the amniotic membran...