A procedure for the preparation of clarified Pawpaw Juice was developed. About 750ml Pawpaw pulp was measured into 2 measuring cylinders A & B of capacity 1 litre heated to 40C, cooled to 20C. 30mls pectinase was added into cylinder A, while 30mls distilled water was added into cylinder B. Enzyme treated sample (A) was allowed to digest for 5hours after which it was heated to 90C for 15 minutes...

BACKGROUND There is some controversy about the extent of changes in different sperm cell features in stored boar semen, especially regarding the potential role of the DNA fragmentation assay for assessment of sperm fertilizing ability. The aim of this study was to assess the effect of time of storage and the dynamic changes in sperm cell characteristics in normospermic boar semen stored in long...

BACKGROUND Adult cancer patients are routinely offered pre-treatment sperm cryopreservation. However, only recently has the welfare of adolescent cancer sufferers gained momentum, including their infertility, and unsurprisingly relatively little is known about their semen quality and feasibility of cryopreservation. METHODS AND RESULTS A total of 238 adolescent cancer patients referred to our...

We have approached the problem of refrigerated storage of epididymal sperm samples from red deer by comparing three options: storing the genital (testicles within the scrotum), diluting the semen in extender or diluting the semen in extender supplemented with an anti-oxidant. Twenty-nine pairs of testes were collected. Spermatozoa from one of each of the pairs were immediately recovered, and di...

T HE livability of stored bull spermatozoa is decreased by aerobic agitation (Prince and Almquist, 1948). Hydrogen peroxide has been implicated in defects of motility, livability and metabolism in spermatozoa of various species (MacLeod, 1943; VanDemark et al., 1949; Tosic and Walton, 1950; Wales et al., 1959). Investigations not involving spermatozoa have demonstrated that exogenous hydrogen p...