FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

this paper seeks to address the issue of alienation from the perspective of mulla sadra. although mulla sadra did not explicitly employed the term alienation, equivalent terms, such as self-forgetfulness, negligence and self-unconsciousness can be found in his works. the outcome of the study is that mulla sadra is pursuing excellence and perfection of human beings through transcendent philosoph...

a case which is observed in the judicial decisions on the patients’ rights is a physician who causes the patient’s death upon his treatment. in a case, the physician doing positional anesthesia observes that the patient is shocked and dies and considering what has been mentioned in the decisions issued by the supreme court, the accused is sentenced to the payment of one complete blood money to ...

As one of the most famous philosophers of the twentieth century, Heidegger has proposed significant ideas about ontology. The undercurrent of his philosophy is the question of being in the world or Dasein. He believes that human is the only creature for whom being in the world is a question and only human is capable of asking about Dasein. Heidegger thinks that authentic poets with their partic...

besides being a literary work, the novel curse of the land (nefrin-e zamin) is a social document. in this great work, al-e ahmad intends to give a social analysis of this own era, and warn his readers against the devastating consequences of modernity. viewed from this perspective, it is regarded as his most sophisticated work representing his social thoughts and theories. in this paper an effor...