1. Efforts to adapt the virus of poliomyelitis to the rabbit organism and to produce poliomyelitis in rabbits by testicular injection and by brain injection after testicular passage produced no evidence that the virus could be adapted in this manner. Suggestive symptoms produced in very young rabbits were duplicated in non-specifically treated and in uninoculated controls. The admixture of a va...

We report the effects of storage conditions and human serum albumin addition to vaccine composition on immunogenic properties an experimental against hemorrhagic fever with renal syndrome. To obtain vaccine, Puumala virus, propagated in Vero cells culture, was concentrated, inactivated beta-propiolactone, purified by chromatography CaptoCore 700 multimodal sorbent (GE Healthcare). The target co...

A comparative study was made of the single radial immunodiffusion test and the classical quantitative precipitin test for determining the amount of precipitable antibodies present in streptococcal groups A and C antisera. The potency of 21 group A and 54 group C antisera was determined by both methods; purified group-specific carbohydrates were used as antigens. The coefficient of correlation b...

The group A streptococcus causes the widest range of disease in humans of all bacterial pathogens. Group A streptococcal diseases are more common in children than adults with diseases ranging from pharyngitis and impetigo to invasive infections and the post-streptococcal sequelae--acute rheumatic fever and acute post-streptococcal glomerulonephritis. The global burden of severe group A streptoc...

Methods Group of 89 patients diagnosed by ophthalmologist included in study. Patients of allergic conjunctivitis included in this study underwent Skin allergy test and specific IgE tests for dust mite allergens. Out of 89 patients 43 received Allergen specific Immunotherapy (SIT) subcutaneous injections with standardized Dermatophgoides farinae (D.f.) and Dermatophagoides pteronyssinus (D.pt.) ...

Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 – 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemoand radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin o...

A pentagonal ring is suggested as the basic structural unit of HCl(H2O)6 and (HCl)2(H2O)6 in solution. Modeled after the X-ray structure of a caged H13O6 compound, it contains bridged H5O2 and Clions, each with a coordination number around four. In the most concentrated HCl solutions, one of the ligands solvating Clis HCl, giving rise to a bichloride moiety. The proposed structure explains qual...

In this paper we address the problem of wave dynamics in presence of concentrated nonlinearities. Given a vector field V on an open subset of C and a discrete set Y ⊂ R with n elements, we define a nonlinear operator ∆V,Y on L (R) which coincides with the free Laplacian when restricted to regular functions vanishing at Y , and which reduces to the usual Laplacian with point interactions placed ...

Case report of a very serious drinking water incident putting up to 50,000 inhabitants of a town near Bonn in North Rhine-Westphalia, Germany at risk. A concentrated solution of highly alkaline water by sodium hydroxide was accidentally washed into the town's drinking water at a pumping station and increased the pH-value of the water to 12. Residents who came into contact with the contaminated ...

An efficient concerted rotation algorithm for use in Monte Carlo statistical mechanics simulations is applied to fold three polypeptides, U(1-17)T9D, alpha(1), and trpzip2, which exhibit native beta-hairpin and alpha-helix folds. The method includes flexible bond and dihedral angles, and a Gaussian bias is applied with driver bond and dihedral angles to optimize the sampling efficiency. Solvati...