Following the pioneering screens for small regulatory RNAs (sRNAs) in Escherichia coli in 2001, sRNAs are now being identified in almost every branch of the eubacterial kingdom. Experimental strategies have become increasingly important for sRNA discovery, thanks to increased availability of tiling arrays and fast progress in the development of high-throughput cDNA sequencing (RNA-Seq). The new...

Source and Description of Probe. A 2.2-kb human cDNA clone for bone morphogenetic protein 5 (BMP5) was excised from the clone U2-16 (Celeste et al., 1990).

The cDNA coding for dog thyrotropin (TSH) receptor was cloned from a dog thyroid library. The cloning procedure was based on the method described previously (Libert et al., 1989). The clone presented here was unambiguously identified as the thyrotropin receptor by expression in oocytes and culture cells (Parnentier et al., 1989).

Source and Description of Clone. A 1.9-kb partial cDNA insert of porcine a-actinin2 in a pBluescript SKphagemid vector was isolated from a swine adult skeletal muscle cDNA lambda ZAP11 library (kindly provided by Charles Louis, University of Minnesota).

Replication of the single-stranded linear DNA genome of parvovirus minute virus of mice (MVM) starts with complementary strand synthesis from the 3'-terminal snap-back telomere, which serves as a primer for the formation of double-stranded replicative form (RF) DNA. This DNA elongation reaction, designated conversion, is exclusively dependent on cellular factors. In cell extracts, we found that...

Short complementary B-form DNA oligomers, 6 to 20 base pairs in length, are found to exhibit nematic and columnar liquid crystal phases, even though such duplexes lack the shape anisotropy required for liquid crystal ordering. Structural study shows that these phases are produced by the end-to-end adhesion and consequent stacking of the duplex oligomers into polydisperse anisotropic rod-shaped ...

Identification of the genes that are specifically expressed in tumor cells but not in normal cells (oncogenes), or vice versa (tumor suppressor genes), is important for understanding the molecular basis of cancer. The differential display technique was applied to compare mRNAs from normal and tumor-derived human mammary epithelial cells, cultured under the same conditions. Complementary DNA fra...