Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3H-isoindol-1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spe...

Lactate dehydrogenase.C. (LDH-C.) from human seminal plasma was purified by 40~60% (NH.)2S0. precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C, were performed with purified LDH-C.. The human LDH-C. exhibits a much higher affinity for pyruvate and a-ketovalerate than lactate. Apparent Km...

In the course of the study of glycolysis in the human red blood cell which is reported in a companion paper (1) new information was collected on the application of column chromatography with anion exchange resins to the isolation of glycolytic intermediates and related compounds. The findings are the subject of this communication. Included in this report are modifications of some analytical met...

pressure (Pe) and temperature (T ). Three-dimensional network shown in two perspectives. Conditions: 25 cm 4.6 mm i.d. packed column, 10 m silica particles; n-pentane. (Reprinted with permission from Lee ML and Markides KE (eds) (1990) Analytical Supercritical Fluid and Extraction. Provo, UT, USA: Chromatography Conferences, Inc.) Jusforgues P (1995) In: Subramanian G (eds) Process Scale Liquid...

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolat...

Glutathione S-transferase rGSTM5* was isolated from rat testis with a combination of glutathione affinity column and reverse-phase column chromatography. The protein was digested with Achromobacter protease I or endoproteinase Arg-C. The peptide fragments were isolated for electrospray MS and N-terminal peptide sequencing analyses. The primary amino acid sequence of rGSTM5* comprises 217 residu...

It is difficult to speak solely about recent developments in a field such as the chromatography of nucleotides, nucleosides and bases since their separation was among the first applications of liquid column chromatography on ionexchange resins (Cohn, 1949). This methodology was subsequently refined, improved and applied to a variety of biochemical and clinical problems by many authors. It was t...

griseoaurantiacus SANK63479. In this paper, we report the isolation procedure and structural elucidation of griseolic acids B2) and C3). Griseolic acids B and C were separated from the mother liquid of griseolic acid A by Sephadex G-10 column chromatography. The mother liquid was dissolved in water and applied on Sephadex G-10 column and developed with water. Two active fractions (griseolic aci...