Seafood spoilage could cause food waste and also serious foodborne disease because of the specific organisms their metabolites. Therefore, it is important to monitor freshness in supply chain. In most cases, has been determined by content volatile base nitrogen, adenosine triphosphate or total viable count. However, these destructive detection methods are tedious time consuming. Hence, highly d...

Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans was determined. However, only the MTT test generated interpretable data when Asp...

Materials and methods Expression of key metabolic markers was assessed by immunohistochemistry in 480 prostate samples and prostate cell lines. The levels of glycolytic metabolism were assessed using commercial colorimetric assays. The effect of the MCT inhibitor and thioridazine was evaluated on cell viability by using the Sulforhodamine B assay. Finally, ultra-structural studies were performe...

The mathematical formulation of calibrating color scanners is presented. The mapping from scanned values to colorimetric values is inherently nonlinear. Calibration required approximating this nonlinear mapping. Neural networks are particularly suited to this task. Performance using an artificial neural network generated LUT is compared to that achieved by other commonly used methods.

BACKGROUND We evaluated the diagnostic performance and safety of combined blind nasotracheal suctioning and non-bronchoscopic mini-bronchoalveolar lavage (mini-BAL) to obtain respiratory secretion specimens from spontaneously breathing, non-intubated patients with infectious pneumonia in intensive care. METHODS Patients suspected of having infectious pneumonia were included prospectively. Thr...

Fourteen commercial soft drinks have been analyzed using colorimetric sensor arrays made from a set of 25 chemically responsive dyes printed on a hydrophobic membrane. Digital imaging of the dye array before and after immersion provides a color change profile as a unique fingerprint for each specific analyte. The digital data library generated was analyzed with statistical and chemometric metho...

The development of new sensitive, cost-effective and user-friendly colorimetric bioassays is in increasing demand to meet the requirement of modern clinical diagnostics and field detection. Herein, a novel iron oxide-to-Prussian blue (PB) nanoparticle (NP) conversion strategy was developed and applied to sensitive colorimetric immunosensing of cancer biomarkers. In a typical sandwich-type immun...

Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional colorimetric absorbance-based methods to measure total protein content. Here we describe the use of 3-(4-carboxybenzoyl)quinoline-2carboxaldehyde (CBQCA) to quantitate...

Multispectral image capture (i.e, more than three channels) facilitates both more accurate tristimulus estimation and possibilities for spectral reconstruction of each scene pixel. A seven-channel camera was assembled using approximately 50 nm bandwidth interference filters, manufactured by Melles Griot, in conjunction with a Kodak Professional DCS 200m digital camera. Multichannel images were ...