We have studied the degradation of type X collagen by human skin fibroblast and rat uterus interstitial collagenases and human 72-kDa type IV collagenase. The interstitial collagenases attacked the native type X helix at two loci, cleaving residues Gly92-Leu93 and Gly420-Ile421, both scissions involving Gly-X bonds of Gly-X-Y-Z-A sequences. However, the human and rat interstitial enzymes displa...

Objective-To study collagenase production in labial salivary glands in patients with Sjogren's syndrome (SS). Methods-Collagenases were localised in labial salivary glands by immunohistochemistry. Collagenase activity against triple helical type I collagen monomers in stimulated saliva was measured using sodium dodecyl sulphate polyacrylamide gel electrophoresis and laser densitometry; tissue i...

Objective—To investigate the eVect of insulin-like growth factor 1 (IGF1) on the release of collagen, and the production and expression of matrix metalloproteinases (MMPs) induced by the proinflammatory cytokine interleukin 1á (IL1á) in combination with oncostatin M (OSM) from bovine nasal cartilage and primary human articular chondrocytes. Methods—Human articular chondrocytes and bovine nasal ...

Oxidative free radicals are one of the most toxic molecules produced in our body. damage accumulates over time, accelerates aging, and contributes to many age-related diseases such as cancer, heart disease Alzheimer’s disease. Our skin is directly exposed damaging environmental factors UV light pollution, which trigger production (FR). Combined with reactive oxygen species formed by skin’s own ...