The northern root-knot nematode, Meloidogyne hapla, is one of the most important nematode pathogens occurring in cold regions. It is a sedentary, biotrophic parasites of plants and overwinter in the soil or in diseased roots. This study showed that the cold tolerance for the second-stage juveniles (J2) of M. hapla was moderate with the 50% survival temperature (S50) of -2.22°C and the fatal tem...

Using one-dimensional hydrodynamic simulations including interstellar heating, cooling, and thermal conduction, we investigate nonlinear evolution of gas flow across galactic spiral arms. We model the gas as a non-self-gravitating, unmagnetized fluid, and follow its interaction with a stellar spiral potential in a local frame comoving with the stellar pattern. Initially uniform gas with density...

During cold periods ectotherms may improve low temperature tolerance via rapid cold hardening (RCH) over a period of hours and/or long-term cold acclimation (LTCA) during days, weeks or months. However, the effect of duration and the major underlying mechanisms of these processes are still not fully understood. In the present study, the molecular and biochemical responses to RCH (1-3 h) and LTC...

The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSPs from Bacillus subtilis during cold shock, we performed genetic as well as fl...

The psychrotrophic bacterium Arthrobacter globiformis SI55 was grown at 4 and 25 degrees C, and the cell protein contents were analyzed by two-dimensional electrophoresis. Cells subjected to cold shocks of increasing magnitude were also analyzed. Correspondence analysis of protein appearance distinguished four groups of physiological significance. Group I contained cold shock proteins (Csps) ov...