Cells react differently to clustered and dispersed DNA double strand breaks (DSB). Little is known about the initial reaction to simultaneous induction of DSBs with different complexities. Here, we used live cell microscopy to analyse the behaviour of 53BP1-GFP (green fluorescence protein) foci formation at DSBs induced in U2OS cells by alpha particles, X-rays or mixed beams over a 75 min perio...

Objective Titanium dioxide (TiO2) is a white pigment which is used in paints, plastics, etc. It is reported to induce ‎oxidative stress and DNA damage. The N-acetylcysteine (NAC) was used to fight oxidative stress-induced ‎damage in various tissues. The aim of this study was to evaluate the toxic effects of TiO2 nanoparticles by oral ‎administration and the protective role of NAC on testes of a...

Progressive DNA damage and worsening aneuploidy are associated with the development of highly aggressive CTCL. We immunostained MF skin lesions for phosphorylated double-strand break repair genes γH2AX 53BP1 phosphorylated/activated ATM (pATM) found that ongoing breaks were frequent within malignant T cells even in clinically stable lesions. IL-6, a product senescent cells, is second most expre...

Upon DNA damage, p53 has been shown to be modified at a number of N-terminal phosphorylation sites including Ser15 and -33. Here we show that phosphorylation is induced as well at a novel site, Ser20. Phosphorylation at Ser15, -20 and -33 can occur within minutes of DNA damage. Interestingly, while the DNA-binding activities of p53 appear to be dispensable, efficient phosphorylation at these th...

Ultraviolet light induces DNA lesions that block the progression of the replication machinery. Several models speculate that the resumption of replication following disruption by UV-induced DNA damage requires regression of the nascent DNA or migration of the replication machinery away from the blocking lesion to allow repair or bypass of the lesion to occur. Both RuvAB and RecG catalyze branch...

Rapid and transient protein acetylation changes in response to DNA damage Comment on: Bennetzen MV, et al. Cell Cycle 2013; 12:1688–95; PMID:23656789; http://dx.doi.org/10.4161/cc.24758 Stephanie Jungmichel and Melanie Blasius; The Novo Nordisk Foundation Center for Protein Research; University of Copenhagen; Copenhagen, Denmark; Danish Cancer Society Research Center; Copenhagen, Denmark; Email...

Background: DNA damage is one of the major consequences of radiation exposure onto the biological systems. A series of compounds including flavanoids were found to render DNA protection against radiation damage. In this study we elucidated the potential of rutin and rutin hydrate to protect plasmid DNA against damage induced by irradiation. Materials and Methods: DPPH and hydroxyl radical scave...

Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus (MN) test were used to detect DNA damages and cytogenetic changes following crude oil expos...